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Methodological Approaches in Experimental Work

  • Vladimir S. Saakov
  • Alexander I. Krivchenko
  • Eugene V. Rozengart
  • Irina G. Danilova
Chapter
  • 468 Downloads

Abstract

Photobiological processes occur under the influence of light and can be registered in ultraviolet (UV), visible, and near-infrared spectral regions. Generally, values of light flux intensity, I, and wavelength, λ, are used in optical measurements. The frequency index \( \overline{\nu} \) is also considered to characterize an absorbed light. Frequency is expressed in reciprocal seconds and represents the ratio of a radiation velocity c to a wavelength λ (in centimeters or nanometers):
$$ \overline{\upnu}=c/\uplambda =3\times {10}^{17}/{\uplambda}_{\mathrm{nm}}, $$
where с = 3 × 1010 сm s−1 or 3 × 1017 nm s−1, the velocity of light. A frequently used index is the wavenumber \( \overline{\nu} \), which is equal to the number of waves in 1 cm. The wavenumber is a reciprocal wavelength expressed in reciprocal centimeters:
$$ \left[{\mathrm{cm}}^{-1}\right]:\overline{\upnu}=1/{\uplambda}_{\mathrm{cm}}={10}^7/{\uplambda}_{\mathrm{nm}} $$
Thus, \( \overline{\nu} \) = 40,000 cm−1, when λ = 250 nm. A number of spectrophotometers have wavenumbers on scale dials. For example, the wavelength of the cadmium red line, as accepted by international agreement, is equal to 6438.4696 Å (angstroms). So 1 Å is 1/6438.4696 of cadmium red line.

Keywords

Saakov Marenko Panel Menu Derivative Spectra Belikov 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Vladimir S. Saakov
    • 1
  • Alexander I. Krivchenko
    • 2
  • Eugene V. Rozengart
    • 2
  • Irina G. Danilova
    • 3
  1. 1.Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of ScienceSaint PetersburgRussia
  2. 2.Inst. of Evolutionary Physiology and Biochem.Russian Academy of ScienceSaint PetersburgRussia
  3. 3.Morbid Anatomy LaboratoryResearch Institute of Medical PrimatologySochi (Adler)Russia

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