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RNAi-Mediated Gene Silencing in the Beta-Lactam Producer Fungi Penicillium chrysogenum and Acremonium chrysogenum

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Genetic Transformation Systems in Fungi, Volume 2

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

RNA silencing represents a powerful approach to test gene function, especially when essential genes with a lethal deletion phenotype are investigated. This phenomenon can be triggered by different systems. One of them gives rise to the formation of double-stranded RNA molecules by means of plasmid pJL43-RNAi (J Microbiol Methods 75:209–218, 2008), which has proven its efficiency in the beta-lactam producing fungi, Penicillium chrysogenum and Acremonium chrysogenum.

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Acknowledgements

 The development of the pJL43-RNAi plasmid was part of the research activity supervised by Professor Juan-Francisco Martín (University of León, Spain) and was supported by grants of the European Union (Eurofung QLRT-1999-00729I) and DSM (Delft, The Netherlands).

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Authors and Affiliations

  1. INBIOTEC (Instituto de Biotecnología de León), León, 24006, Spain

    Carlos García-Estrada D.V.M., Ph.D. & Ricardo V. Ullán Ph.D.

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  1. Carlos García-Estrada D.V.M., Ph.D.
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  2. Ricardo V. Ullán Ph.D.
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Correspondence to Carlos García-Estrada D.V.M., Ph.D. .

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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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García-Estrada, C., Ullán, R.V. (2015). RNAi-Mediated Gene Silencing in the Beta-Lactam Producer Fungi Penicillium chrysogenum and Acremonium chrysogenum . In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 2. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10503-1_9

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