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Genetic Transformation Systems in Fungi, Volume 2 pp 225–243Cite as

Genetic and Genomic Manipulations in Aspergillus niger

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Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Well-characterized selectable markers and promoters have been developed for the efficient transformation of Aspergillus niger for the production of proteins and for strain construction. Genetic transformation of this organism has been used widely for the production and secretion of homologous proteins and proteins from animals, plants, bacteria, and other fungi. The genome of A. niger harbors hundreds of genes encoding gene regulators, solute transporters, and biomass-degrading enzymes. Genome-wide and genome-based studies have substantially enhanced our understanding of the metabolic network, the roles of transcription regulators, and the identity of secondary metabolites. These studies have also led to the rational design of strains for the improved production and secretion of proteins and metabolites. With a finished genome sequence and robust transformation systems, A. niger is well positioned to become a synthetic biology platform.

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Acknowledgements

This work was supported by Genome Canada, Génome Québec, and the NSERC Strategic Bioconversion Network. We thank Dr. Ian Reid for critical reading of the manuscript.

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Authors and Affiliations

  1. Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, Canada, H4B1R6

    Adrian Tsang, Annie Bellemare & Corinne Darmond

  2. DSM Biotechnology Center, Delft, The Netherlands

    Janny Bakhuis

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  1. Adrian Tsang
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Correspondence to Adrian Tsang .

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  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Tsang, A., Bellemare, A., Darmond, C., Bakhuis, J. (2015). Genetic and Genomic Manipulations in Aspergillus niger . In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 2. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10503-1_20

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