Abstract
Traditional methods for automated and high-throughput morphological analysis of filamentous fungi can be time-consuming and difficult. Here, two suitable image acquisition methods and subsequent image and data processing steps are presented. The acquisition methods are based on imaging flow cytometry and whole-slide microscopy. Guidelines for developing customized image and data analysis routines for satisfying individual research requirements are presented.
Keywords
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Cox P, Paul G, Thomas C (1998) Image analysis of the morphology of filamentous micro-organisms. Microbiology 144(Pt 4):817–827
Hnisz D, Tscherner M, Kuchler K (2011) Morphological and molecular genetic analysis of epigenetic switching of the human fungal pathogen Candida albicans. Methods Mol Biol 734:303–315
Krabben P, Nielsen J. (1998). Modeling the mycelium morphology of Penicillium species in submerged cultures. In: Schügerl K, ed. Relation between morphology and process performances [Internet]. Springer: Berlin. [cited 2013 Apr 25]. p. 125–52. Available from: http://link.springer.com/chapter/ 10.1007/BFb0102281
Krull R, Wucherpfennig T, Esfandabadi ME, Walisko R, Melzer G, Hempel DC et al (2013) Characterization and control of fungal morphology for improved production performance in biotechnology. J Biotechnol 163(2):112–123
MATLAB and Image Processing Toolbox Release. (2013a). Natick: The MathWorks, Inc.
Pomati F, Nizzetto L (2013) Assessing triclosan-induced ecological and trans-generational effects in natural phytoplankton communities: a trait-based field method. Ecotoxicology 22(5):779–794
Posch AE, Spadiut O, Herwig C (2012) A novel method for fast and statistically verified morphological characterization of filamentous fungi. Fungal Genet Biol 49(7):499–510
Posch AE, Herwig C, Spadiut O (2013) Science-based bioprocess design for filamentous fungi. Trends Biotechnol 31(1):37–44
Acknowledgements
The authors would like to thank Sandoz GmbH for providing the strains and guidance. Financial support was provided by the Austrian research funding association (FFG) under the scope of the COMET program within the research network “Process Analytical Chemistry (PAC)” (contract # 825340).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Golabgir, A., Ehgartner, D., Neutsch, L., Posch, A.E., Sagmeister, P., Herwig, C. (2015). Imaging Flow Cytometry and High-Throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 2. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10503-1_17
Download citation
DOI: https://doi.org/10.1007/978-3-319-10503-1_17
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-10502-4
Online ISBN: 978-3-319-10503-1
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)