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An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS)

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Book cover Animal Cell Culture

Part of the book series: Cell Engineering ((CEEN,volume 9))

Abstract

The insect cell-baculovirus protein expression vector system (BEVS) has gained increasing attention as more of its products are approved for human use. However, the system has been relevant for many years, being used for the manufacturing of recombinant veterinary vaccines, as a workhorse in the research laboratory, as an important tool for new drug discovery and as an important source of commercial materials and reagents for research. In this chapter, the key elements that should be considered for the design of a productive BEVS process are discussed, along with a presentation of the state of the art of the system.

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Acknowledgments

We are thankful to Enrique Paz for his artwork. Technical support by Rosa Román and Roberto Rodríguez. Financial support by PAPIIT UNAM IT-200113 and IT-210214.

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Authors and Affiliations

  1. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

    Laura A. Palomares & Octavio T. Ramírez

  2. Diagnóstico Clínico y Molecular DICLIM, Cuernavaca, Morelos, Mexico

    Mauricio Realpe

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  1. Laura A. Palomares
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  2. Mauricio Realpe
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  3. Octavio T. Ramírez
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Correspondence to Laura A. Palomares .

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  1. University College Dublin, Dublin, Ireland

    Mohamed Al-Rubeai

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Palomares, L.A., Realpe, M., Ramírez, O.T. (2015). An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS). In: Al-Rubeai, M. (eds) Animal Cell Culture. Cell Engineering, vol 9. Springer, Cham. https://doi.org/10.1007/978-3-319-10320-4_15

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