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Cell Line Development

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Animal Cell Culture

Part of the book series: Cell Engineering ((CEEN,volume 9))

Abstract

The majority of processes to manufacture biotherapeutics for use in humans are based on mammalian cell lines. Established cell lines are modified by recombinant DNA technologies to produce specific proteins. Such technologies together with cell culture processes have been improved over decades for today’s manufacturing processes.

This chapter describes gene expression approaches to create mammalian hosts that produce the protein of choice. It focuses on methods that are currently applied or have been developed in the last years as well as technologies that are thought to be relevant for future applications. While these methods are directed towards high, stable and predictable production of the secreted protein they also form the basis for further modifications that lead to robust manufacturing processes through improvement of cellular properties.

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  1. Department of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany

    Hansjoerg Hauser

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  1. University College Dublin, Dublin, Ireland

    Mohamed Al-Rubeai

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Hauser, H. (2015). Cell Line Development. In: Al-Rubeai, M. (eds) Animal Cell Culture. Cell Engineering, vol 9. Springer, Cham. https://doi.org/10.1007/978-3-319-10320-4_1

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