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Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareri)

  • Kostyantyn Dmytruk
  • Andriy Sibirny
Chapter
  • 2.2k Downloads
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Genetic transformation is a primary prerequisite for efficient gene manipulation of a target microorganism. Here, we describe the method of electrotransformation (electroporation) of the flavinogenic yeast Candida famata (Candida flareri), available selective markers for the selection of the transformants and insertional mutagenesis for this yeast species.

Keywords

Transformation system Insertional mutagenesis Insertion cassette Selective marker Candida famata (Candida flareri) 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Kostyantyn Dmytruk
    • 1
  • Andriy Sibirny
    • 1
    • 2
  1. 1.Department of Molecular Genetics and Biotechnology, Institute of Cell BiologyNational Academy of Sciences of UkraineLvivUkraine
  2. 2.Department of Biotechnology and MicrobiologyUniversity of RzeszówRzeszówPoland

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