Chemical Transformation of Candida albicans
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Genetic transformation is an essential technique for studying gene function in the yeast Candida albicans. Although less efficient than spheroplasting- or electroporation-techniques, the lithium acetate-based protocol described below is easy to perform, requires basic lab equipment and reliably yields good number of the expected transformants. This method can also be adapted for high-throughput analysis purposes.
KeywordsCandida albicans Transformation Integration Lithium acetate Heat-shock
We wish to thank Anne Neville and Mélanie Legrand for critical reading of the manuscript.
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