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Electroporation Mediated DNA Transformation of Filamentous Fungi

  • B. N. Chakraborty
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

The electroporation offers a simple, rapid, reproducible, and efficient transformation method for various species of filamentous fungi without making protoplast or without the need to use of toxic chemicals. This procedure is directly applicable to sporulating species and can also be used with either mycelia or non-conidiating species. Selection of the age for conidia and germination stage, nature of mycolytic enzyme(s), duration of treatment, choice of selectable markers and selection medium are to be considered as critical factors while yielding high transformants. Multiple copies of plasmids can integrate at unlinked sites and thus provide the potential for increased yield of the desired product. Repeat induced point mutation has proved to be useful in the mutagenesis of specific DNA fragments in vivo. The filamentous fungi provide potential for innovation to identify new promoters and regulatory sequences and the application of genetic transformation promises a healthy future for fungal biotechnology.

Keywords

Electroporation Co-transformation β-glucuronidase Heat shock protein RIP Filamentous fungi 

Notes

Acknowledgements

Financial assistance received from the Department of Biotechnology, Ministry of Science and Technology, Government of India in the form of Long-term Biotechnology Associateship program under dynamic guidance of Professor Manju Kapoor at Cellular, Molecular and Microbial Biology Division, Department of Biological Sciences, The University of Calgary, Canada is gratefully acknowledged.

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© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • B. N. Chakraborty
    • 1
  1. 1.Department of BotanyUniversity of North BengalSiliguriIndia

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