Transformation of Saccharomyces cerevisiae: Spheroplast Method

  • Shigeyuki Kawai
  • Kousaku MurataEmail author
Part of the Fungal Biology book series (FUNGBIO)


During the latter half of the 1970s, first spheroplasts transformation method was established in the yeast Saccharomyces cerevisiae. With the advent of synthetic biology and genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of S. cerevisiae.


Spheroplasts Saccharomyces cerevisiae Yeast artificial chromosome Synthetic bacterial genome Infectious prion 


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Graduate School of AgricultureKyoto UniversityUjiJapan

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