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Transformation of Mucor circinelloides f. lusitanicus Protoplasts

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Genetic Transformation Systems in Fungi, Volume 1

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Since the first report of a Mucor circinelloides transformation system, many things have been improved by small changes that either simplify the original procedure, or use new technologies. Today, people working on this fungus have alternative methods of transformation that allow them choosing the most suitable one for their purposes. Preparation and manipulation of protoplasts has become a key step to a more efficient transformation procedure for this fungus, which is used as model species in the Mucorales to develop fermentation processes for the production of β-carotene, other carotenoids, and essential oils like gamma-linolenic acid and biodiesel.

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Acknowledgements

We want to thank everyone who have played with M. circinelloides during the last 25 years and have established important observations and small-step modifications on the original transformation procedure, which have converted this fungus into a real model organism.

Author information

Authors and Affiliations

  1. Department of Genetics and Microbiology, University of Murcia, Murcia, 30100, Spain

    Victoriano Garre Ph.D.

  2. Department of Biotechnology, Gadea Biopharma, Parque Tecnológico de León, C/ Nicostrato Vela s/n, León, 24009, Spain

    José Luis Barredo Ph.D.

  3. Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Avda. Campo Charro s/n, Salamanca, 37007, Spain

    Enrique A. Iturriaga Ph.D.

Authors
  1. Victoriano Garre Ph.D.
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  2. José Luis Barredo Ph.D.
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  3. Enrique A. Iturriaga Ph.D.
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Corresponding author

Correspondence to José Luis Barredo Ph.D. .

Editor information

Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Garre, V., Barredo, J.L., Iturriaga, E.A. (2015). Transformation of Mucor circinelloides f. lusitanicus Protoplasts. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_4

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