Transformation of Mucor circinelloides f. lusitanicus Protoplasts
- 2.2k Downloads
Since the first report of a Mucor circinelloides transformation system, many things have been improved by small changes that either simplify the original procedure, or use new technologies. Today, people working on this fungus have alternative methods of transformation that allow them choosing the most suitable one for their purposes. Preparation and manipulation of protoplasts has become a key step to a more efficient transformation procedure for this fungus, which is used as model species in the Mucorales to develop fermentation processes for the production of β-carotene, other carotenoids, and essential oils like gamma-linolenic acid and biodiesel.
KeywordsMucor circinelloides Protoplasts Cell-wall lytic enzymes PEG-mediated transformation Electroporation DNA integration
We want to thank everyone who have played with M. circinelloides during the last 25 years and have established important observations and small-step modifications on the original transformation procedure, which have converted this fungus into a real model organism.
- Iturriaga EA, Velayos A, Eslava AP, Álvarez MI (2001) The genetics and molecular biology of carotenoid biosynthesis in Mucor. Recent Res Dev Genet 1:79–92Google Scholar
- Schipper MAA (1976) On Mucor circinelloides, Mucor racemosus and related species. Stud Mycol 12:1–40Google Scholar
- Suárez T (1985) Obtención de protoplastos y transformación en Phycomyces blakesleeanus. PhD thesis, University of Salamanca, Salamanca, SpainGoogle Scholar