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Transformation of Mucor circinelloides f. lusitanicus Protoplasts

  • Victoriano Garre
  • José Luis BarredoEmail author
  • Enrique A. Iturriaga
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Since the first report of a Mucor circinelloides transformation system, many things have been improved by small changes that either simplify the original procedure, or use new technologies. Today, people working on this fungus have alternative methods of transformation that allow them choosing the most suitable one for their purposes. Preparation and manipulation of protoplasts has become a key step to a more efficient transformation procedure for this fungus, which is used as model species in the Mucorales to develop fermentation processes for the production of β-carotene, other carotenoids, and essential oils like gamma-linolenic acid and biodiesel.

Keywords

Mucor circinelloides Protoplasts Cell-wall lytic enzymes PEG-mediated transformation Electroporation DNA integration 

Notes

Acknowledgements

We want to thank everyone who have played with M. circinelloides during the last 25 years and have established important observations and small-step modifications on the original transformation procedure, which have converted this fungus into a real model organism.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Victoriano Garre
    • 1
  • José Luis Barredo
    • 2
    Email author
  • Enrique A. Iturriaga
    • 3
  1. 1.Department of Genetics and MicrobiologyUniversity of MurciaMurciaSpain
  2. 2.Department of BiotechnologyGadea BiopharmaLeónSpain
  3. 3.Area de Genética, Departamento de Microbiología y GenéticaUniversidad de SalamancaSalamancaSpain

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