TALEN-Based Genome Editing in Yeast
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The development of nuclease-mediated Genome Editing represents a significant technological advance and may prove to be as important as PCR and DNA sequencing. Regardless, this technology puts the power of genome re-orchestration into the hands of research scientists for the first time and may lead to a paradigm shift in many biological fields. We present background information, so the reader has a greater understanding for the basis of this technology. We also discuss TAL effector nuclease (TALEN) as the latest engineered nuclease tool, its implementation and uses and present a yeast-based protocol that may be helpful in transitioning this technology to other fungal species.
KeywordsTAL effector nuclease (TALEN) Zinc finger nuclease Meganuclease Non-homologous end-joining (NHEJ) Homologous recombination (HR) Genetic engineering Gene editing Genome editing
The work on TALEN technology development and application in the Yang lab and the Spalding lab have been funded by several funding agencies. The authors wish to acknowledge the US National Science Foundation (Award number 1238189 to B.Y. and MCB-0952323 to M.H.S.) and the US Department of Energy’s Advanced Research Projects Agency-Energy Program (DEAR0000010 to M.H.S.) and Office of Science, Basic Energy Science Division. (DE-FG02-12ER16335 to M.H.S.)
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