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Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle

  • Aurelijus ZimkusEmail author
  • Audrius Misiūnas
  • Arūnas Ramanavičius
  • Larisa Chaustova
Chapter
  • 2.2k Downloads
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Capability of taking up exogenous DNA of yeast Saccharomyces cerevisiae cells was studied by lipophilic cations accumulation and FT-IR spectroscopy. The increase in permeability of the yeast cells treated with Li+ ions was observed. The changes in the cell wall structure detected by FT-IR spectra were associated with transformability of yeast cells. The presence of a population of cells with buds with a higher permeability to TPP cations was determined. Relationship between transformation efficiency of yeast Saccharomyces cerevisiae and cell cycle was determined. Yeast cells in the S-phase of cell cycle showed an enhanced perception to exogenous DNA. An increase was associated with physiological and permeability properties of yeast cells. The phenomenon of natural competence is supposed to occur in yeast cells.

Keywords

Saccharomyces cerevisiae Cell cycle Natural competence Permeability FT-IR 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Aurelijus Zimkus
    • 1
    • 2
    Email author
  • Audrius Misiūnas
    • 3
    • 4
  • Arūnas Ramanavičius
    • 2
    • 5
  • Larisa Chaustova
    • 6
  1. 1.Department of Biochemistry and Molecular BiologyVilnius UniversityVilniusLithuania
  2. 2.Center for Physical Sciences and TechnologyVilniusLithuania
  3. 3.Department of Organic ChemistryCenter for Physical Sciences and TechnologyVilniusLithuania
  4. 4.Department of BiopharmaceuticalCentre of Innovative MedicineVilniusLithuania
  5. 5.Center of Nanotechnology and Materials Science—NanoTechnas, Faculty of ChemistryVilnius UniversityVilniusLithuania
  6. 6.Department of Bioelectrochemistry and Biospectroscopy Institute of BiochemistryVilnius UniversityVilniusLithuania

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