Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics
- 2.2k Downloads
Spontaneous yeast competence for exogenous DNA uptake is powered by naturally occurring cell traits/processes controlled by genetic and environmental factors. Therefore, yeast transformability is defined as a complex, quantitative genetic trait which may have contributed to fungal evolution. Such contemplation has elucidated comprehensive mechanisms of natural competence involved in spontaneous yeast transformation under environmental conditions. Moreover, several recent reports have identified many genes and/or entire cell processes responsible for the phenomenon in yeast Saccharomyces cerevisiae. However, this is hardly the case for many non-Saccharomyces species significantly important to humanity. Therefore, knowledge on pathways/mechanisms of eukaryotic competence is still only partial, inconclusive and not comprehensive. For instance, although we can line up all the necessary steps leading to transformation, we still lack fundamental knowledge to logically assemble them. Obviously, many important questions still remain open to a more systematic and targeted approach that will paradigmatically broaden our knowledge on the subject.
KeywordsYeast Saccharomyces Transformation Natural competence Pathways and Mechanisms
I wish to thank to my postdoc boss Dr. Krunoslav Brčić-Kostić (Ruđer Bošković Institute) for critical reading of the manuscript and generous support and to M.Sc. Juraj Bergman (Ruđer Bošković Institute) for polishing the English. I would also like to acknowledge the Editors for editing the manuscript in such a way that my original ideas and thoughts are now expressed more profoundly. Finally, I specially wish to acknowledge Professor Kousaku Murata (Graduate School of Agriculture, Kyoto University) for his ideas on fungal transformation grossly influenced mine.
- Gietz RD, Woods RA (2001) Genetic transformation of yeast. Biotechniques 30:816–820, 822–826, 828 passimGoogle Scholar
- Tomlin GC, Hamilton GE, Gardner DCJ, Walmsley RM, Stateva LI, Oliver SG (2000) Suppression of sorbitol dependence in a strain bearing a mutation in the SRB1/PSA1/VIG9 gene encoding GDP-mannose pyrophosphorylase by PDE2 overexpression suggests a role for the Ras/cAMP signal-transduction pathway in the control of yeast cell-wall biogenesis. Microbiology 146:2133–2146PubMedGoogle Scholar