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Transformation of Fungi Using Shock Waves

  • Miguel A. Gómez-LimEmail author
  • Denis Magaña Ortíz
  • Francisco Fernández
  • Achim M. Loske
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Genetic transformation of fungi faces major challenges. Most standard methods have a low frequency of transformation, problems of reproducibility, and are not suitable for high throughput experimentation. Additionally, there are many species of fungi that have proved recalcitrant to transformation by biolistics, electroporation, or agrobacterium transformation. We have developed a new method, based on the use of underwater shock waves that is highly efficient, simple, widely applicable, and not dependent on one particular type of tissue. Intact spores, conidia, or mycelia can be used. The method consists of the application of a few hundred shock waves to cell suspensions. Acoustic cavitation, i.e., the growth and violent collapse of microbubbles contained in the suspension, is responsible for a transient cell permeabilization. Several fungal species including, Aspergillus niger, Fusarium oxysporum, Phanerochaete chrysosporium, Trichoderma reesei, and Mycosphaerella fijiensis have been transformed successfully by this method. As an example, the methodology to transform Aspergillus niger is described in this chapter. Information on the basics of shock wave-mediated transformation of fungi is also included. Our main conclusion is that shock wave-mediated transformation is an attractive alternative for efficient fungal transformation.

Keywords

Filamentous fungi Genetic transformation Shock waves Biotechnology Shock wave-mediated transformation 

Notes

Acknowledgements

The authors would like to thank Nancy Coconi, Claudia León, Elizabeth Ortiz, René Preza, Ángel Luis Rodríguez, and Guillermo Vázquez for technical assistance. This work was supported by DGAPA, UNAM Grant Number IN108410, and CONACYT Grant Number 22655.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Miguel A. Gómez-Lim
    • 1
    Email author
  • Denis Magaña Ortíz
    • 1
  • Francisco Fernández
    • 2
  • Achim M. Loske
    • 2
  1. 1.Department of Plant Genetic EngineeringCentro de Investigación y de Estudios Avanzados del IPNIrapuatoMexico
  2. 2.Centro de Física Aplicada y Tecnología AvanzadaUniversidad Nacional Autónoma de MéxicoJuriquillaMexico

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