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Protoplast Transformation for Genome Manipulation in Fungi

  • Aroa Rodriguez-Iglesias
  • Monika SchmollEmail author
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Fungi are organisms, which can serve as models for investigation of physiological processes and additionally, they have a high potential for industrial applications. In order to leverage their full potential, their genomes are modified for analysis of gene functions, elucidation of metabolic processes and also for their adjustment to biotechnological processes for efficient production of enzymes and metabolites. One of the most prominent methods for genome manipulation by transformation is protoplast transformation. In this review, we give an overview on prerequisites for protoplasting and transformation as well as methods and conditions for every single step as applied in diverse fungi. Thereby, we aim to provide a basis for troubleshooting with established methods and for development of new protocols to be applied to species for which transformation has not yet been achieved. Thereafter, we briefly summarize tools for enhanced efficiency of transformation, which have been applied in combination with protoplast transformation.

Keywords

Genome modification Protoplast transformation Protoplasting Cell wall degradation Gene deletion Complementation Functional genomics 

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© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Health and Environment—BioresourcesAIT Austrian Institute of TechnologyTullnAustria

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