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High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method

  • R. Daniel GietzEmail author
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

The ability to transform DNA into an organism is an essential element that aids greatly in many molecular and genetic studies. In Saccharomyces cerevisiae numerous methods can be used to accomplish this task; however, the LiAc/ssDNA/PEG methods are presented here. Methods for various applications are listed including a method for transformation in 96 well microtiter plate format as well as a method for the production and transformation of frozen competent yeast cells.

Keywords

Saccharomyces cerevisiae DNA uptake Lithium acetate Polyethylene glycol Transformation 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Biochemistry and Medical GeneticsUniversity of ManitobaWinnipegCanada

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