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Glass-Bead and Agrobacterium-Mediated Genetic Transformation of Fusarium oxysporum

  • Manish Pareek
  • Mahak Sachdev
  • Meenakshi Tetorya
  • Manchikatla V. RajamEmail author
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

We have described the protocols for glass-bead and Agrobacterium-mediated genetic transformation for a filamentous fungus, Fusarium oxysporum. These transformation protocols are easy, rapid, efficient, and cost-effective for transformation of F. oxysporum and can be adopted for other filamentous fungi, including recalcitrant fungi. These methods provide efficient tools for functional analysis of genes over the conventional methods.

Keywords

Glass-beads Agrobacterium Genetic transformation Fusarium oxysporum Fungi 

Notes

Acknowledgements

MVR is grateful to the Department of Biotechnology (DBT), Department of Science and Technology (DST) (under FIST Program), University Grants Commission (under SAP Program), and Delhi University (under DST PURSE Program), New Delhi, India for generously funding research programs. MP, MS, and MT are thankful to the Council of Scientific and Industrial Research (CSIR) and University Grant Commission (UGC) for fellowships.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Manish Pareek
    • 1
  • Mahak Sachdev
    • 1
  • Meenakshi Tetorya
    • 1
  • Manchikatla V. Rajam
    • 1
    Email author
  1. 1.Department of GeneticsUniversity of Delhi South CampusNew DelhiIndia

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