Agrobacterium tumefaciens- Mediated Transformation of Pucciniomycotina Red Yeasts
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Red yeasts belong to the subphylum Pucciniomycotina, one of the three major lineages in the Basidiomycete fungi. These yeasts, commonly found as saprophytes in the environment, have unique features that make them important for academia, industry, agriculture, and occasionally human health. To understand the genetic basis behind biological processes of interest, the availability of molecular tools for gene mutation plays a crucial role. Here we outline how to perform recently developed protocols of Agrobacterium tumefaciens-mediated transformation to Pucciniomycotina red yeasts. This method can be applied to generate libraries of T-DNA insertional mutants that can be screened for phenotypes of interest, or for targeted gene replacement.
KeywordsPucciniomycotina Sporobolomyces sp. Agrobacterium tumefaciens-mediated transformation (AMT) Auxotrophic marker Insertional mutagenesis
Our research on the Pucciniomycotina fungi has been supported by the University of Missouri Research Board, the US National Science Foundation, and the National Institutes of Health.
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