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Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment

  • Eiji SakuradaniEmail author
  • Hiroshi Kikukawa
  • Seiki Takeno
  • Akinori Ando
  • Sakayu Shimizu
  • Jun Ogawa
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Uracil auxotrophs derived from the Zygomycete Mortierella alpina through spontaneous mutation were transformed by biolistic particle bombardment. Effective biolistic transformation of intact spores was achieved using the Bio-Rad PDS-1000/He system. Transformation frequencies of up to several colonies per μg of circular DNA were obtained under optimized conditions.

Keywords

Mortierella alpina Transformation Biolistic particle bombardment Oleaginous fungus Fatty acid 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Eiji Sakuradani
    • 1
    Email author
  • Hiroshi Kikukawa
    • 2
  • Seiki Takeno
    • 2
  • Akinori Ando
    • 2
  • Sakayu Shimizu
    • 2
  • Jun Ogawa
    • 2
  1. 1.Institute of Technology and ScienceThe University of TokushimaTokushimaJapan
  2. 2.Division of Applied Life Sciences, Graduate School of AgricultureKyoto UniversityKyotoJapan

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