Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment
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Uracil auxotrophs derived from the Zygomycete Mortierella alpina through spontaneous mutation were transformed by biolistic particle bombardment. Effective biolistic transformation of intact spores was achieved using the Bio-Rad PDS-1000/He system. Transformation frequencies of up to several colonies per μg of circular DNA were obtained under optimized conditions.
KeywordsMortierella alpina Transformation Biolistic particle bombardment Oleaginous fungus Fatty acid
- Amano H, Shinmen Y, Akimoto K, Kawashima H, Amachi T, Shimizu S, Yamada H (1992) Chemotaxonomic significance of fatty acid composition in the genus Mortierella (Zygomycetes, Mortierellaceae). Mycotaxon 94(2):257–265Google Scholar
- Ando A, Sumida Y, Negoro H, Suroto DA, Ogawa J, Sakuradani E, Shimizu S (2009) Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding. Appl Environ Microbiol 75(17):5529–5535PubMedCrossRefPubMedCentralGoogle Scholar
- Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S (2004a) Cloning and sequencing of the ura3 and ura5 genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4. Biosci Biotechnol Biochem 68(2):277–285PubMedCrossRefGoogle Scholar