Use of the Biolistic Particle Delivery System to Transform Fungal Genomes
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Biolistic delivery of transforming DNA into fungal genomes, especially when performed on uninucleate haploid conidia, has proven successful in bypassing the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.
KeywordsBiolistic particle delivery system Filamentous fungi Fungal conidia Trichoderma reesei Fungal genome
The authors would like to thank the Australian Research Council for funding parts of this research.
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