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Use of the Biolistic Particle Delivery System to Transform Fungal Genomes

  • V. S. Junior Te’o
  • K. M. Helena NevalainenEmail author
Chapter
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Part of the Fungal Biology book series (FUNGBIO)

Abstract

Biolistic delivery of transforming DNA into fungal genomes, especially when performed on uninucleate haploid conidia, has proven successful in bypassing the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.

Keywords

Biolistic particle delivery system Filamentous fungi Fungal conidia Trichoderma reesei Fungal genome 

Notes

Acknowledgments

The authors would like to thank the Australian Research Council for funding parts of this research.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • V. S. Junior Te’o
    • 1
  • K. M. Helena Nevalainen
    • 1
    Email author
  1. 1.Department of Chemistry and Biomolecular SciencesMacquarie UniversitySydneyAustralia

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