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Deposition and Characterization of Laccase Thin Films Obtained by Matrix Assisted Pulsed Laser Evaporation

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Sensors

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 319))

Abstract

Matrix Assisted Pulsed Laser Evaporation (MAPLE) was used as an alternative technique within strategies for enzyme immobilization to produce Laccase thin films.

In order to characterize Laccase thin films, suitable deposition substrates were used. Laccase layers deposited onto silicon were investigated by Fourier Transform InfraRed Spectroscopy (FTIR) and Atomic Force Microscopy (AFM) to study molecular structure and surface morphology of Laccase thin films, respectively. Moreover, to estimate the amount of deposited enzyme, Quartz Cristal Microbalance (QCM) electrodes modified with Laccase films were used. Finally, glass and screen printed carbon electrodes were used as deposition substrates to test by colorimetric assay and amperometry the activity of the Laccase deposited. Experimental results have demonstrated that MAPLE enables the Laccase deposition that retains 10 % of the initial activity.

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Acknowledgments

This research was supported by FESR Basilicata 2007/2013 as an investment in LDFS project.

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Authors and Affiliations

  1. Consiglio Nazionale delle Ricerche, Istituto di Metodologie per l’Analisi Ambientale, Tito Scalo (PZ), Italy

    Nunzia Cicco

  2. Consiglio Nazionale delle Ricerche, Istituto di struttura della Materia, Tito Scalo (PZ), Italy

    Antonio Morone

  3. Consiglio Nazionale delle Ricerche, Istituto di struttura della Materia, Tito Scalo (PZ), Italy

    Maria Verrastro

  4. Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Potenza, Italy, Potenza, Italy

    Maria Verrastro

  5. Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Bucharest, Romania

    Maria Dinescu, Andreea Matei & Bogdana Mitu

  6. Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli Studi di Foggia, Foggia, Italy

    Diego Centonze

Authors
  1. Nunzia Cicco
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  2. Antonio Morone
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  3. Maria Verrastro
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  4. Maria Dinescu
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  5. Andreea Matei
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  6. Bogdana Mitu
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  7. Diego Centonze
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Corresponding author

Correspondence to Maria Verrastro .

Editor information

Editors and Affiliations

  1. Faculty of Bioscience and Technologies for Food Agriculture and Environment, University of Teramo, Mosciano S.A., Teramo, Italy

    Dario Compagnone

  2. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  3. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. University of Cassino, Cassino, Italy

    Giovanni Betta

  5. CNR, Head of Inst. of Microelectronics a, Lecce, Italy

    Pietro Siciliano

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© 2015 Springer International Publishing Switzerland

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Cicco, N. et al. (2015). Deposition and Characterization of Laccase Thin Films Obtained by Matrix Assisted Pulsed Laser Evaporation. In: Compagnone, D., Baldini, F., Di Natale, C., Betta, G., Siciliano, P. (eds) Sensors. Lecture Notes in Electrical Engineering, vol 319. Springer, Cham. https://doi.org/10.1007/978-3-319-09617-9_9

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  • DOI: https://doi.org/10.1007/978-3-319-09617-9_9

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09616-2

  • Online ISBN: 978-3-319-09617-9

  • eBook Packages: EngineeringEngineering (R0)

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