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Sensors pp 53–58Cite as

Optical Characterization of Heavy Metal-Binding Proteins Bioconjugation on Porous Silicon Devices

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 319))

Abstract

Biosensors are hot topic in recent years because of their versatility to different fields. Porous silicon devices are optical transducer sensitive to biological and chemical species which penetrate inside the pores after a proper functionalization with specific bioprobes. In this work, we reported a functionalization approach to obtain optical biosensors for heavy metal detection. Oligopeptides, namely Phytochelatins, were selected due to their ability to detect heavy metal ions. Spectroscopic reflectometry and Fourier transform infrared spectroscopy were used as characterization techniques. Data show successful and repeatable functionalization process. These results are very good starting step in development of heavy metal ions optical biosensors.

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Acknowledgments

Work is supported by Italian National Operative Program PON01_01525.

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Authors and Affiliations

  1. Institute for Microelectronics and Microsystems, National Research Council, Unit of Naples, Via P. Castellino 111, 80131, Napoli, Italy

    Jane Politi, Principia Dardano, Mario Iodice, Ilaria Rea & Luca De Stefano

  2. Department of Chemistry, University of Naples, “Federico II”, Via Cinthia, 80128, Napoli, Italy

    Jane Politi

Authors
  1. Jane Politi
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  2. Principia Dardano
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  3. Mario Iodice
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  4. Ilaria Rea
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  5. Luca De Stefano
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Corresponding author

Correspondence to Jane Politi .

Editor information

Editors and Affiliations

  1. Faculty of Bioscience and Technologies for Food Agriculture and Environment, University of Teramo, Mosciano S.A., Teramo, Italy

    Dario Compagnone

  2. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  3. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. University of Cassino, Cassino, Italy

    Giovanni Betta

  5. CNR, Head of Inst. of Microelectronics a, Lecce, Italy

    Pietro Siciliano

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© 2015 Springer International Publishing Switzerland

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Politi, J., Dardano, P., Iodice, M., Rea, I., Stefano, L. (2015). Optical Characterization of Heavy Metal-Binding Proteins Bioconjugation on Porous Silicon Devices. In: Compagnone, D., Baldini, F., Di Natale, C., Betta, G., Siciliano, P. (eds) Sensors. Lecture Notes in Electrical Engineering, vol 319. Springer, Cham. https://doi.org/10.1007/978-3-319-09617-9_10

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  • DOI: https://doi.org/10.1007/978-3-319-09617-9_10

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09616-2

  • Online ISBN: 978-3-319-09617-9

  • eBook Packages: EngineeringEngineering (R0)

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