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Scanning Electron Microscopy View of In Vitro Intraneural Injections

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Abstract

Scanning electron microscopy (SEM) technique allows identification of features from anatomical structures that are traversed by the tip of the needle during an accidental dural puncture. Lesions to axons enclosed in injured fascicles may occur in conjunction with damage to blood vessels within the intrafascicular tissue. As a result of blood vessel tissue repair and hematoma reabsorption, variable degrees of fibrosis may affect areas in the proximity of axons. The study of distortions in “used” needle tips, lesions caused to nerve fascicles, and the diameters of blood vessels may aid in the understanding of the mechanisms leading to tissue damage and repair after accidental dural puncture.

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References

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Authors and Affiliations

  1. Department of Clinical Medical Sciences and Institute of Applied Molecular Medicine, School of Medicine, University of CEU San Pablo, Madrid, Spain

    Miguel Angel Reina MD, PhD

  2. Department of Anesthesiology, Madrid-Montepríncipe University Hospital, Madrid, Spain

    Miguel Angel Reina MD, PhD

  3. Human Anatomy and Embryology Unit, School of Medicine, University of Barcelona, Barcelona, Spain

    Xavier Sala-Blanch MD

  4. Department of Anesthesiology and Critical Care, Clinic Hospital, Barcelona, Spain

    Xavier Sala-Blanch MD

Authors
  1. Miguel Angel Reina MD, PhD
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  2. Xavier Sala-Blanch MD
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Corresponding author

Correspondence to Miguel Angel Reina MD, PhD .

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Editors and Affiliations

  1. Department of Anesthesiology, Department of Clinical Medical Sciences and Institute of Applied Molecular Medicine, School of Medicine, University of CEU San Pablo, Madrid, Spain, and Madrid-Montepríncipe University Hospital, Madrid, Spain

    Miguel Angel Reina

  2. Department of Surgical Specialties, Department of Anesthesia, Critical Care, and Pain Management, General University Hospital, School of Medicine, University of Valencia, Valencia, Spain

    José Antonio De Andrés

  3. College of Physicians and Surgeons, Department of Anesthesiology, St. Luke’s-Roosevelt Hospital Center, New York School of Regional, Anesthesia (NYSORA), New York, NY, USA, and Columbia University, New York, New York, USA

    Admir Hadzic

  4. Laboratory of Surgical NeuroAnatomy, Human Anatomy and Embryology Unit, School of Medicine, University of Barcelona, Barcelona, Spain

    Alberto Prats-Galino

  5. Department of Anesthesiology and Critical Care, Human Anatomy and Embryology Unit, School of Medicine, University of Barcelona, Barcelona, Spain, and Clinic Hospital, Barcelona, Spain

    Xavier Sala-Blanch

  6. Discipline of Anesthesiology, The University of Queensland and Royal Brisbane and Women’s Hospital, Brisbane, Queensland, Australia

    André A.J. van Zundert

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© 2015 Springer International Publishing Switzerland

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Reina, M.A., Sala-Blanch, X. (2015). Scanning Electron Microscopy View of In Vitro Intraneural Injections. In: Reina, M., De Andrés, J., Hadzic, A., Prats-Galino, A., Sala-Blanch, X., van Zundert, A. (eds) Atlas of Functional Anatomy for Regional Anesthesia and Pain Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-09522-6_16

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  • DOI: https://doi.org/10.1007/978-3-319-09522-6_16

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09521-9

  • Online ISBN: 978-3-319-09522-6

  • eBook Packages: MedicineMedicine (R0)

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