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Applications of the Spin-Trapping Method in Radiation Biology

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Applications of EPR in Radiation Research

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Abstract

Ionizing radiation produces many kinds of free radicals in biologically important molecules through direct or indirect actions. In this chapter we have shown that a method that combined ESR, spin trapping and chromatographic separation made it possible to identify free radicals induced by direct ionization as well as OH-radical reactions in nucleic-acid related and protein-related compounds. In addition, combination with enzymatic digestion expanded the potential of this method to detect free radical production in molecules with high molecular weight such as polynucleotides, RNA and DNA, and polypeptides and proteins. The effects of ionizing radiation on biological molecules underlie the primary processes involved in radiation biology. ESR combined with spin trapping remains a useful method for clarifying these processes in a living organism.

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Authors and Affiliations

  1. Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, 060–0818, Sapporo, Japan

    Mikinori Kuwabara & Osamu Inanami

  2. Laboratory of Biophysics, School of Science and Technology, Meiji University, 214–8571, Kawasaki, Japan

    Wakako Hiraoka

Authors
  1. Mikinori Kuwabara
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  2. Wakako Hiraoka
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  3. Osamu Inanami
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Correspondence to Mikinori Kuwabara .

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Editors and Affiliations

  1. The Emeritus Academy Linköping University, Linköping, Sweden

    Anders Lund

  2. Hiroshima University, Higashi-Hiroshima, Japan

    Masaru Shiotani

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Kuwabara, M., Hiraoka, W., Inanami, O. (2014). Applications of the Spin-Trapping Method in Radiation Biology. In: Lund, A., Shiotani, M. (eds) Applications of EPR in Radiation Research. Springer, Cham. https://doi.org/10.1007/978-3-319-09216-4_9

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