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New Frontiers of Multidisciplinary Research in STEAM-H (Science, Technology, Engineering, Agriculture, Mathematics, and Health) pp 279–293Cite as

Direct Differentiation of Human Pluripotent Stem Cells into Advanced Spermatogenic Cells: In Search of an In Vitro System to Model Male Factor Infertility

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Abstract

Differentiation of stem cells into spermatogenic lineages in vitro provides a unique window into the biological mechanisms responsible for driving pluripotent stem cells into essential progeny—haploid spermatids and viable sperm—as well as provides an innovative approach for determining novel root causes for male infertility. Our recent work outlined a novel approach for differentiating human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) into advanced spermatogenic lineages including haploid spermatids with correct parent-of-origin genomic imprints on two loci. The work described here in this chapter provides a foundation for building a true in vitro model for human spermatogenesis with which to model, diagnose and potentially treat male factor infertility.

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Authors and Affiliations

  1. Department of Cell Biology, Laboratory of Translational Cell Biology, Emory University School of Medicine, 615 Michael St, Whitehead Biomedical Research Building RM405H, Atlanta, GA, 30322, USA

    Charles A. Easley IV

  2. Department of OB/GYN and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Calvin R. Simerly & Gerald Schatten

  3. Magee Womens Research Institute, Pittsburgh Development Center, 204 Craft Ave Pittsburgh, Pittsburgh, PA, 15213, USA

    Calvin R. Simerly & Gerald Schatten

Authors
  1. Charles A. Easley IV
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  2. Calvin R. Simerly
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  3. Gerald Schatten
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Correspondence to Charles A. Easley IV .

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Editors and Affiliations

  1. Dept of Mathematics & Computer Sciences, Virginia State University, Petersburg, Virginia, USA

    Bourama Toni

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Easley, C.A., Simerly, C.R., Schatten, G. (2014). Direct Differentiation of Human Pluripotent Stem Cells into Advanced Spermatogenic Cells: In Search of an In Vitro System to Model Male Factor Infertility. In: Toni, B. (eds) New Frontiers of Multidisciplinary Research in STEAM-H (Science, Technology, Engineering, Agriculture, Mathematics, and Health). Springer Proceedings in Mathematics & Statistics, vol 90. Springer, Cham. https://doi.org/10.1007/978-3-319-07755-0_12

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