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The Role of Reactive Oxygen Species in ASM-Induced Disease Resistance in Apple Fruit

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Post-harvest Pathology

Part of the book series: Plant Pathology in the 21st Century ((ICPP,volume 7))

Abstract

Blue mould caused by Penicillium expansum is one of the most important postharvest diseases of apple fruit. The present study was to evaluate how disease resistance in apple fruit (cv. Fuji) was affected by the dipping of acibenzolar-S-methyl (ASM) and diphenylene iodonium (DPI), a NADPH oxidase specific inhibitor. Lesion diameter on the fruit inoculated with P. expansum was significantly (P ≤ 0.05) decreased by dipping with 0.1 g/L ASM. Decreased lesion development was associated with the accumulation of hydrogen peroxide (H2O2), release of superoxide anion (O2 −), enhancement activities of NADPH oxidase (NOX), superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR). Antioxidants content including ascorbic acid (AsA) and reduce glutathione (GSH) was also induced by ASM treatment. While catalase (CAT) activity was inhibited by ASM dipping. Compared with ASM treated fruit, fruit treated with DPI prior to ASM treatment exhibited bigger lesion diameter. Moreover, DPI treatment inhibited ASM-induced H2O2 and O2 − accumulation, the increase of the activities of NOX, SOD, APX, GR and content of AsA and GSH. These results suggest that pretreatment with DPI prevented accumulation of ROS induced by ASM and showed serious disease symptoms, which showed the important role of ROS in ASM-induced resistance in apple fruit.

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Acknowledgement

This work was supported by the National Natural Science Foundation of China (31160405), Doctoral Initial Funding of Bohai University (bsqd201405) and Funding of Food Safety Key Lab of Liaoning Province (LNSAKF2013021).

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Food Safety, Bohai University, No 19 Keji Road, Songshan New District, Jinzhou, 121013, People’s Republic of China

    Yonghong Ge & Canying Li

  2. College of Food Science and Engineering, Gansu Agricultural University, No 1 Yingmencun, Anning District, Lanzhou, 730070, People’s Republic of China

    Huiwen Deng, Yang Bi & Yaoyao Liu

Authors
  1. Yonghong Ge
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  2. Huiwen Deng
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  3. Yang Bi
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  4. Canying Li
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  5. Yaoyao Liu
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Correspondence to Yonghong Ge .

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Editors and Affiliations

  1. Postharvest Science of Fresh Produce, Agricultural Research Organization Dept., Bet-Dagan, Israel

    Dov Prusky

  2. in campo agro-ambientale, Università degli Studi di Torino Competenza per l'Innovazione, Grugliasco, Torino, Italy

    Maria Lodovica Gullino

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Ge, Y., Deng, H., Bi, Y., Li, C., Liu, Y. (2014). The Role of Reactive Oxygen Species in ASM-Induced Disease Resistance in Apple Fruit. In: Prusky, D., Gullino, M. (eds) Post-harvest Pathology. Plant Pathology in the 21st Century, vol 7. Springer, Cham. https://doi.org/10.1007/978-3-319-07701-7_4

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