Abstract
The economic losses caused by post-harvest pathogens of apple and pear can still reach 25 %. There is currently an increasing demand to develop sustainable methods to control these post-harvest pathogens. Biocontrol agents are interesting candidates to answer this demand. Nevertheless, their commercial development is sometimes hampered by a low or non-reliable efficacy comparing to fungicide treatments. Fundamental research on the mode of action of the BCA and of its ecological fitness could help to overcome that phenomenon. This chapter reviews the progresses made during two decades to understand the mode of action and the ecological niche of two BCA, Pichia anomala strain K and Candida oleophila strain O. These advances required the combination of various methodologies (in vitro and in situ) and techniques (microbiology, microscopy, genome characterization, transcriptome, proteome, gene disruption…) which are summarized here. Importantly, the practical impact of these discoveries to improve the efficacy of the biopesticide is also highlighted.
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References
Alani E, Cao L, Kleckner N (1987) A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116(4):541–545
Box GEP, Behnken DW (1960) Some new three level design for study of quantitative variables. Technometerics 2:21
Friel D, Vandenbol M, Jijakli MH (2005) Genetic characterization of the yeast Pichia anomala (strain K), an antagonist of postharvest diseases of apple. J Appl Microbiol 98(3):783–788
Friel D et al (2007) Separate and combined disruptions of two exo-β-1,3-glucanase genes decrease the efficiency of Pichia anomala (strain K) biocontrol against Botrytis cinerea on apple. Mol Plant Microbe Interact 20(4):371–379
Grevesse C, Lepoivre P, Jijakli MH (2003) Characterization of the exoglucanase-encoding gene PaEXG2 and study of its role in the biocontrol activity of Pichia anomala strain K. Phytopathology 93(9):1145–1152
Jijakli MH (2011) Pichia anomala in biocontrol for apples: 20 years of fundamental research and practical applications. Antonie Van Leeuwenhoek 99:13
Jijakli H, Lepoivre P (1993) Biological control of post-harvest Botrytis cinerea and Penicillium on apples. IOBC/WPRS Bull 16:5
Jijakli MH, Lepoivre P (1998) Characterization of an exo-β-1,3-glucanase produced by Pichia anomala strain K, antagonist of Botrytis cinerea on apples. Phytopathology 88(4):335–343
Kwasiborski A et al (2012) Biocontrol proteomics: development of an in situ interaction model and a protein extraction method for a proteomic study of the inhibiting mechanisms of Pichia anomala against Botrytis cinerea. BioControl 57(6):837–848
Kwasiborski A, Bajji M, Renaut J, Delaplace P, Jijakli MH (2014) Identification of metabolic pathways expressed by Pichia anomala KH6 in the presence of the pathogen Botrytis cinerea on apple: new possible targets for biocontrol improvement. PLOS one 9(3), March 2014
Lahlali R, Jijakli MH (2009) Enhancement of the biocontrol agent Candida oleophila (strain O) survival and control efficiency under extreme conditions of water activity and relative humidity. Biol Control 51(3):403–408
Lahlali R et al (2006) Effect of incubation temperature and relative humidity on lesion diameter of Botrytis cinerea Pers. and Penicillium expansum Link. on apple fruits. Commun Agric Appl Biol Sci 71(3 Pt B):1159–1166
Lahlali R et al (2008) A Box-Behnken design for predicting the combined effects of relative humidity and temperature on antagonistic yeast population density at the surface of apples. Int J Food Microbiol 122(1–2):100–108
Lahlali R et al (2009) Assessment of Pichia anomala (strain K) efficacy against blue mould of apples when applied pre- or post-harvest under laboratory conditions and in orchard trials. Eur J Plant Pathol 123(1):37–45
Lahlali R, Brostaux Y, Jijakli MH (2011a) Control of apple blue mold by the antagonistic yeast pichia anomala strain K: screening of UV protectants for preharvest application. Plant Dis 95(3):311–316
Lahlali R, Raffaele B, Jijakli MH (2011b) UV protectants for Candida oleophila (strain O), a biocontrol agent of postharvest fruit diseases. Plant Pathol 60(2):288–295
Massart S, Jijakli MH (2006) Identification of differentially expressed genes by cDNA-amplified fragment length polymorphism in the biocontrol agent Pichia anomala (Strain Kh5). Phytopathology 96(1):80–86
Massart S, Jijakli HM (2007) Use of molecular techniques to elucidate the mechanisms of action of fungal biocontrol agents: a review. J Microbiol Methods 69(2):229–241
Steyaert JM et al (2003) Genetic basis of mycoparasitism: a mechanism of biological control by species of Trichoderma. N Z J Crop Hort Sci 31(4):281–291
Wilson CL, Wisnieswski ME (1994) Biological control of post-harvest diseases: theory and practice. CRC Press, London
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Sebastien, M., Jijakli, M.H. (2014). Pichia anomala and Candida oleophila in Biocontrol of Postharvest Diseases of Fruits: 20 Years of Fundamental and Practical Research. In: Prusky, D., Gullino, M. (eds) Post-harvest Pathology. Plant Pathology in the 21st Century, vol 7. Springer, Cham. https://doi.org/10.1007/978-3-319-07701-7_10
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DOI: https://doi.org/10.1007/978-3-319-07701-7_10
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