Skip to main content
SpringerLink
Log in
Book cover

Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases and Their Biomimetic Complexes pp 13–64Cite as

Experimental

  • Chapter
  • First Online:

  • 296 Accesses

Part of the book series: Springer Theses ((Springer Theses))

Abstract

This chapter will give an introduction of the experimental methods used in enzyme expression and purification and the synthesis of the compounds discussed in this thesis.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Notes

  1. 1.

    Parts of this chapter appeared in: L.J. Daumann et al., “Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation” J Inorg Biochem. 2014, 131, 1–7; L.J. Daumann et al., “Asymmetric Zinc(II) Complexes as Functional and Structural Models for Phosphoesterases” Dalton Trans. 2013; 42, 9574–9584; L.J. Daumann et al., “Dinuclear Cobalt(II) Complexes as Metallo-β-lactamase Mimics” Eur. J. Inorg. Chem. 2013, 17, 3082–3089. L.J. Daumann et al. “Synthesis, Magnetic Properties and Phosphoesterase Activity of Binuclear Cobalt(II) Complexes” Inorg. Chem. 2013, 52 (4), 2029–2043; L.J. Daumann et al., “Promiscuity Comes at a Price: Catalytic Versatility vs. Efficiency in Different Metal Ion Derivatives of the Potential Bioremediator GpdQ” BBA—Proteins and Proteomics 2013, 1834, 1, 425–432; L.J. Daumann et al., “Cadmium(II) Complexes; Mimics of Organophosphate Pesticide Degrading Enzymes and Metallo-β-lactamases” Inorg. Chem. 2012, 51, 7669-7681; L.J. Daumann et al., “The Role of Zn-OR and Zn-OH Nucleophiles and the Influence of p-Substituents in the Reactions of Binuclear Phosphatase Mimetics” Dalton Trans. 2012, 41, 1695–1708. L.J. Daumann et al., “Spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes” Dalton Trans. 2014, 43, 910–928.”.

References

  1. G. Ambrosi, M. Formica, V. Fusi, L. Giorgi, M. Micheloni, Coord. Chem. Rev. 252, 1121–1152 (2008)

    Article  CAS  Google Scholar 

  2. R. Robson, Aust. J. Chem. 23, 2217–2224 (1970)

    Article  CAS  Google Scholar 

  3. R. Robson, Inorg. Nucl. Chem. Lett. 6, 125–128 (1970)

    Article  CAS  Google Scholar 

  4. R.E. Mirams, S.J. Smith, K.S. Hadler, D.L. Ollis, G. Schenk, L.R. Gahan, J. Biol. Inorg. Chem. 13, 1065–1072 (2008)

    Article  CAS  Google Scholar 

  5. R.R. Buchholz, M.E. Etienne, A. Dorgelo, R.E. Mirams, S.J. Smith, S.Y. Chow, L.R. Hanton, G.B. Jameson, G. Schenk, L.R. Gahan, J. Chem. Soc., Dalton Trans. 6045–6054 (2008)

    Google Scholar 

  6. L.R. Gahan, S.J. Smith, A. Neves, G. Schenk, Eur. J. Inorg. Chem. 19, 2745–2758 (2009)

    Article  Google Scholar 

  7. S.J. Smith, A. Casellato, K.S. Hadler, N. Mitić, M.J. Riley, A.J. Bortoluzzi, B. Szpoganicsz, G. Schenk, A. Neves, L.R. Gahan, J. Biol. Inorg. Chem. 12, 1207–1220 (2007)

    Article  CAS  Google Scholar 

  8. H. Carlsson, M. Haukka, E. Nordlander, Inorg. Chem. 43, 5681–5687 (2004)

    Article  CAS  Google Scholar 

  9. H. Carlsson, E. Nordlander, M. Jarenmark, C. R. Chim. 10, 433–462 (2007)

    Article  Google Scholar 

  10. M. Jarenmark, E. Csapo, J. Singh, S. Wockel, E. Farkas, F. Meyer, M. Haukka, E. Nordlander, J. Chem. Soc., Dalton Trans. 39, 8183–8194 (2010)

    Article  CAS  Google Scholar 

  11. M. Jarenmark, S. Kappen, M. Haukka, E. Nordlander, J. Chem. Soc., Dalton Trans 993–996 (2008)

    Google Scholar 

  12. S. Albedyhl, Marie T. Averbuch-Pouchot, C. Belle, B. Krebs, Jean L. Pierre, E. Saint-Aman, S. Torelli, Eur. J. Inorg. Chem. 2001, 1457–1464 (2001)

    Google Scholar 

  13. J.A. Larrabee, W.R. Johnson, A.S. Volwiler, Inorg. Chem. 48, 8822–8829 (2009)

    Article  CAS  Google Scholar 

  14. J.A. Larrabee, S.A. Chyun, A.S. Volwiler, Inorg. Chem. 47, 10499–10508 (2008)

    Article  CAS  Google Scholar 

  15. A. Mangalum, R.C. Smith, Tetrahedron 65, 4298–4303 (2009)

    Article  CAS  Google Scholar 

  16. X. Dong, X. Wang, Y. He, Z. Yu, M. Lin, C. Zhang, J. Wang, Y. Song, Y. Zhang, Z. Liu, Y. Li, Z. Guo, Chem. Eur. J. 16, 14181–14189 (2010)

    Google Scholar 

  17. T. Routasalo, J. Helaja, J. Kavakka, A.M.P. Koskinen, Eur. J. Org. Chem. 2008, 3190–3199 (2008)

    Article  Google Scholar 

  18. T. Inomata, K. Shinozaki, Y. Hayashi, H. Arii, Y. Funahashi, T. Ozawa, H. Masuda, Chem. Commun. (Camb.) 392–394 (2008)

    Google Scholar 

  19. H. Sharghi, M.A. Nasseri, K. Niknam, J. Org. Chem. 66, 7287 (2001)

    Article  CAS  Google Scholar 

  20. A. Zinke, R. Ott, E. Leggewie, A. Hassanein, G. Zankl, Monatshefte für Chemie / Chemical Monthly 87, 552–559 (1956)

    Article  CAS  Google Scholar 

  21. V. Boehmer, F. Marschollek, L. Zetta, J. Org. Chem. 52, 3200–3205 (1987)

    Article  CAS  Google Scholar 

  22. R.T. Paine, Y.C. Tan, X.M. Gan, Inorg. Chem. 40, 7009–7013 (2001)

    Article  CAS  Google Scholar 

  23. J.d. Mendoza, P.M. Nieto, P. Prados and C. Sánchez, Tetrahedron, 46, 671–682 (1990)

    Google Scholar 

  24. S. Striegler, M. Dittel, Inorg. Chem. 44, 2728–2733 (2005)

    Article  CAS  Google Scholar 

  25. E.F. Elslager, E.L. Benton, F.W. Short, F.H. Tendick, J. Am. Chem. Soc. 78, 3453–3457 (1956)

    Article  CAS  Google Scholar 

  26. Bruker, TopSpin 2.0 Software for NMR data processing

    Google Scholar 

  27. M. Hesse, H. Meier, B. Zeeh, Spectroscopic Methods in Organic Chemistry (G. Thieme, NY, 1997)

    Google Scholar 

  28. D.F. Evans, J. Chem. Soc., 1959 (2003)

    Google Scholar 

  29. E.M. Schubert, J. Chem. Educ. 69, 62 (1992)

    Article  CAS  Google Scholar 

  30. G.A. Bain, J.F. Berry, J. Chem. Educ. 85, 532–536 (2008)

    Article  CAS  Google Scholar 

  31. H. Sakiyama, Inorg. Chim. Acta 360, 715–716 (2007)

    Article  CAS  Google Scholar 

  32. Thermo-Scientific, Grams/AI 9.0 Software

    Google Scholar 

  33. M. J. Riley, VTVH 2.1.1 Program for the simulation and fitting variable temperature—variable field MCD spectra (2008)

    Google Scholar 

  34. H. Adamsky, AOMX Program (1996)

    Google Scholar 

  35. F. Ely, K.S. Hadler, N. Mitić, L.R. Gahan, D.L. Ollis, N.M. Plugis, M.T. Russo, J.A. Larrabee, G. Schenk, J. Biol. Inorg. Chem. 16, 777–787 (2011)

    Article  CAS  Google Scholar 

  36. G.M. Sheldrick, SHELXL97: Program for the refinement of crystal structures (1997)

    Google Scholar 

  37. L.J. Farrugia, J. Appl. Crystallogr. 32, 837–838 (1999)

    Article  CAS  Google Scholar 

  38. R.A. Peralta, A.J. Bortoluzzi, B. de Souza, R. Jovito, F.R. Xavier, R.A. A. Couto, A. Casellato, F. Nome, A. Dick, L.R. Gahan, G. Schenk, G.R. Hanson, F.C.S. de Paula, E.C. Pereira-Maia, S.d.P. Machado, P.C. Severino, C. Pich, T. Bortolotto, H. Terenzi, E.E. Castellano, A. Neves, M.J. Riley, Inorg. Chem. 49, 11421–11438 (2010)

    Google Scholar 

  39. I.H. Segel, Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Wiley, NY, 1975)

    Google Scholar 

  40. N.V. Kaminskaia, B. Spingler, S.J. Lippard, J. Am. Chem. Soc. 122, 6411–6422 (2000)

    Article  CAS  Google Scholar 

  41. N.V. Kaminskaia, C. He, S.J. Lippard, Inorg. Chem. 39, 3365–3373 (2000)

    Article  CAS  Google Scholar 

  42. C.S. Ltd, CasaXPS: Processing Software for XPS, AES, SIMS and More (2009)

    Google Scholar 

  43. R.V. Jones, L. Godorhazy, N. Varga, D. Szalay, L. Urge, F. Darvas, J. Comb. Chem. 8, 110–116 (2006)

    Article  CAS  Google Scholar 

  44. K.S. Hadler, N. Mitic, S.H. Yip, L.R. Gahan, D.L. Ollis, G. Schenk, J.A. Larrabee, Inorg. Chem. 49, 2727–2734 (2010)

    Article  CAS  Google Scholar 

  45. C.J. Jackson, K.S. Hadler, P.D. Carr, A.J. Oakley, S. Yip, G. Schenk, D.L. Ollis, Acta crystallogr. F 64, 681–685 (2008)

    Article  CAS  Google Scholar 

  46. J.M. Walker, The Proteomics Protocols Handbook (Humana Press, Totowa, 2005)

    Book  Google Scholar 

  47. V.M. D’Souza, B. Bennett, A.J. Copik, R.C. Holz, Biochemistry 39, 3817–3826 (2000)

    Article  Google Scholar 

  48. K.S. Hadler, E.A. Tanifum, S.H. Yip, N. Mitić, L.W. Guddat, C.J. Jackson, L.R. Gahan, K. Nguyen, P.D. Carr, D.L. Ollis, A.C. Hengge, J.A. Larrabee, G. Schenk, J. Am. Chem. Soc. 130, 14129–14138 (2008)

    Article  CAS  Google Scholar 

  49. H. Tsue, K. Enyo, K.-I. Hirao, Org. Lett. 2, 3071–3074 (2000)

    Article  CAS  Google Scholar 

  50. G.-C. Sun, Z.-H. He, Z.-J. Li, X.-D. Yuan, Z.-J. Yang, G.-X. Wang, L.-F. Wang, C.-R. Liu, Molecules 6, 1001–1005 (2001)

    Article  CAS  Google Scholar 

  51. T. Aoki, T. Kaneko, N. Maruyama, A. Sumi, M. Takahashi, T. Sato, M. Teraguchi, J. Am. Chem. Soc. 125, 6346–6347 (2003)

    Article  CAS  Google Scholar 

  52. W. Müller, P. Kipfer, D.A. Lowe, S. Urwyler, Helv. Chim. Acta 78, 2026–2035 (1995)

    Article  Google Scholar 

  53. A.S. Borovik, V. Papaefthymiou, L.F. Taylor, O.P. Anderson, L. Que, J. Am. Chem. Soc. 111, 6183–6195 (1989)

    Article  CAS  Google Scholar 

  54. E. Díez-Barra, J.M. Fraile, J.I. García, E. García-Verdugo, C.I. Herrerías, S.V. Luis, J.A. Mayoral, P. Sánchez-Verdú, J. Tolosa, Tetrahedron Asymmetry 14, 773–778 (2003)

    Article  Google Scholar 

  55. S. Carloni, V. Borzatta, L. Moroni, G. Tanzi, G. Sartori, R. Maggi, Catalysts Based on Metal Complexes for the Synthesis of Optically Active Chrysanthemic Acid (2005), p. 55 (Patent WO2005123254A1)

    Google Scholar 

  56. C.A. Bunton, S.J. Farber, J. Org. Chem. 34, 767–772 (1969)

    Article  CAS  Google Scholar 

  57. R.S. Molday, Canadian Patents and Development Limited (CA) Magnetic Iron-Dextran Microspheres (1984) (Patent 4452773)

    Google Scholar 

  58. B.-F. Pan, F. Gao, H.-C. Gu, J. Colloid Interface Sci. 284, 1–6 (2005)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia

    Lena Josefine Daumann

Authors
  1. Lena Josefine Daumann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lena Josefine Daumann .

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Daumann, L.J. (2014). Experimental. In: Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases and Their Biomimetic Complexes. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-06629-5_2

Download citation

Publish with us

Policies and ethics

Search

Navigation