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Biosensors Based on Urease Adsorbed on Nickel, Platinum, and Gold Conductometric Transducers Modified with Silicalite and Nanozeolites

  • Ivan S. KucherenkoEmail author
  • Oleksandr O. Soldatkin
  • Berna Ozansoy Kasap
  • Burcu Akata Kurç
  • Volodymir G. Melnyk
  • Lyudmila M. Semenycheva
  • Sergei V. Dzyadevych
  • Alexei P. Soldatkin
Conference paper
Part of the Springer Proceedings in Physics book series (SPPHY, volume 156)

Abstract

This work describes urease-based conductometric biosensors that were created using nontypical method of urease immobilization via adsorption on micro- and nanoporous particles: silicalite and nanocrystalline zeolites Beta (BEA) and L. Conductometric transducers with nickel, gold, and platinum interdigitated electrodes were used. Active regions of the nickel transducers were modified with microparticles using two procedures—spin coating and drop coating. Gold and platinum transducers were modified with silicalite using drop coating since it was more effective. Scanning electron microscopy was used to evaluate effectiveness of these procedures. The procedure of spin coating produced more uniform layers of particles (and biosensors had good reproducibility of preparation), but it was more complicated, drop coating was easier and led to formation of a bulk of particles; thus, biosensors had bigger sensitivity but worse reproducibility of preparation. Urease was immobilized onto transducers modified with particles by physical adsorption. Analytical characteristics of the obtained biosensors for determination of urea (calibration curves, sensitivity, limit of detection, linear concentration range, noise of responses, reproducibility of signal during a day, and operational stability during 3 days) were compared. Biosensors with all three particles deposited by spin coating showed similar characteristics; however, silicalite was a bit more effective. Biosensors based on nickel transducers modified by drop coating had better characteristics in comparison with modification by spin coating (except reproducibility of preparation). Transducers with gold electrodes showed best characteristics while creating biosensors, platinum electrodes were slightly inferior to them, and nickel electrodes were the worst.

Keywords

Spin Coating Platinum Electrode Nickel Electrode Interdigitated Electrode Biosensor Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors gratefully acknowledge the financial support of this study by Project European IRSES-318524-NANODEV. Furthermore, this study was partly supported by NASU in the frame of Scientific and Technical Program “Sensor devices for medical-ecological and industrial purposes: metrology and trial performance.”

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Ivan S. Kucherenko
    • 1
    • 2
    Email author
  • Oleksandr O. Soldatkin
    • 1
    • 3
  • Berna Ozansoy Kasap
    • 4
  • Burcu Akata Kurç
    • 4
    • 5
  • Volodymir G. Melnyk
    • 6
  • Lyudmila M. Semenycheva
    • 6
  • Sergei V. Dzyadevych
    • 1
    • 3
  • Alexei P. Soldatkin
    • 1
    • 3
  1. 1.Laboratory of Biomolecular Electronics, Institute of Molecular Biology and GeneticsNational Academy of Sciences of UkraineKyivUkraine
  2. 2.Institute of BiologyTaras Shevchenko National University of KyivKyivUkraine
  3. 3.Institute of High TechnologiesTaras Shevchenko National University of KyivKyivUkraine
  4. 4.Central LaboratoryMiddle East Technical UniversityAnkaraTurkey
  5. 5.Micro and Nanotechnology DepartmentMiddle East Technical UniversityAnkaraTurkey
  6. 6.Department of Electrical and Magnetic Measurements, Institute of ElectrodynamicsNational Academy of Sciences of UkraineKyivUkraine

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