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Platinum Nanoparticles with Adsorptive Layer of Chlorella vulgaris Polysaccharides Inactivate Tumor Cells of Ascitic Ehrlich Carcinoma, Ovarian Cancer and Leukemia

  • V. R. Estrela-LlopisEmail author
  • A. V. Chevichalova
  • N. A. Tregubova
  • E. D. Shishko
  • P. M. Litvin
Conference paper
Part of the Springer Proceedings in Physics book series (SPPHY, volume 156)

Abstract

In comparison (with) nanogold, not much work on the application of nanoplatinum in cancer therapy is known. The purpose of this work is to investigate the aggregation of platinum nanoparticles with different cancer cells and its effects on cells’ vital activity.

The “green” synthesis of platinum nanoparticles from hexachloroplatinic acid solution was conducted, using as a reducing agent ascorbic acid for platinum nanoparticles, and as a reducing stabilizer—polysaccharides of Chlorella for the same nanoparticles modified by the polysaccharide adsorption layer. Here, we take into consideration the known properties of Chlorella’s polysaccharides as immunostimulators, inhibitors of proliferation, and activators of apoptosis of cancer cells.

It was studied the interaction of NP PtPS with cancer cells (ascitic Ehrlich carcinoma, ovarian cancer A2780 human line, leukemia - transformed β-lymphocytes-cells Namalwa line, and for comparison - with lymphocytes of healthy human) on the basis of a complex of methods, including studies of heterocoagulation of cells and particles and kinetics of cell death (cytotoxic effect).

This study showed that for the first 20 minutes of contact were died over 50% cells of ovarian cancer and Ehrlich carcinoma. For leukemic cells, such time is 2 h. The selectivity of NP PtPS action was established—more than 90 % of healthy lymphocytes maintain their viability after 2 h of contact. Bioconjugates of platinum nanoparticles with polysaccharides can be the basis of the perspective drug for cancer therapy.

Keywords

Ovarian Cancer Adsorption Layer Platinum Nanoparticles Human Ovarian Cancer Cell Ascitic Ehrlich Carcinoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Dykman L, Khlebtsov N (2012) Gold nanoparticles in biomedical applications: recent advances and perspectives. Chem Soc Rev 41:2256–2282CrossRefGoogle Scholar
  2. 2.
    Gamaleja N, Lesnyak G, Prokopenko I (2007) Photobiology and photomedicine 7:67 (rus).Google Scholar
  3. 3.
    Huang X, Prashant K, El-Sayed IH, El-Sayed MA (2007) Gold nanoparticles and nanorods in medicine: from cancer diagnostics to photothermaltherapy.Nanomedicine 2(5):681–693CrossRefGoogle Scholar
  4. 4.
    Krutyakov Y, Kudrinskiy A, Olenin A, Lisichkin G (2008) Synthesis and properties of silver nanoparticles: advances and prospects. Russ Chem Rev 77(3):233–257ADSCrossRefGoogle Scholar
  5. 5.
    Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA (2012) The golden age: gold nanoparticles for biomedicine. Chem Soc Rev 41:2740–2779CrossRefGoogle Scholar
  6. 6.
    Jain PK, Huang X, El-Sayed IH, El-Sayed MA (2007) Review of some interesting surface plasmon resonance-enhanced properties of noble metal nanoparticles and their applications to biosystems. Plasmonics 2:107–111CrossRefGoogle Scholar
  7. 7.
    Khlebtsov BN, Zharov VP, Melnikov AG, Tuchin VV, Khlebtsov NG (2006) Optical amplification of photothermal therapy with gold nanoparticles and nanoclusters. Nanotechnology 17(20):5167–5179ADSCrossRefGoogle Scholar
  8. 8.
    Pissuwan D, Niidome T, Cortie MB (2011) The forthcoming applications of gold nanoparticles in drug and gene delivery systems. J Controlled Release 149:65–71CrossRefGoogle Scholar
  9. 9.
    Shi J, Xiao Z, Kamaly N, Farokhzad OC (2011) Self-assembled targeted nanoparticles. Acc Chem Res 44(10):1123–1134CrossRefGoogle Scholar
  10. 10.
    Abalde-Cela S, Aldeanueva-Potel P, Mateo-Mateo C et al (2010) Surface-enhanced Raman scattering biomedical application of plasmonic colloidal particles. J Royal Soc Interface 7:435–450CrossRefGoogle Scholar
  11. 11.
    Vigderman L, Zubarev ER (2012) Therapeutic platforms based on gold nanoparticles and their covalent conjugates with drug molecules. Adv Drug Deliv Rev 65:663–676 (1 Rev)CrossRefGoogle Scholar
  12. 12.
    Bhattacharya R, Mukherjee P (2008) Biological properties of “naked” metal nanoparticles. Adv Drug Delivery Rev 60:1289–1306MathSciNetCrossRefGoogle Scholar
  13. 13.
    Yatsimirskii KB (1976) Problems of bioneorganicheskoy chemistry. Moscow: MirGoogle Scholar
  14. 14.
    Hikosaka K et al (2008) Platinum nanoparticles have an activity similar to mitochondrial NADH: ubiquinone oxidoreductase. Colloids Surf B 66:195–200CrossRefGoogle Scholar
  15. 15.
    Asharani PV, Xinyi N, Hande M, Valiyaveettil S (2010) DNA damage and p53-mediated growth arrest in human cells treated with platinum nanoparticles. Nanomedicine 5(1):51CrossRefGoogle Scholar
  16. 16.
    Kralovec JA (2005) Chlorella composition having high molecular weight polysaccharides and polysaccharide complexes. Patent US No. 6974576 (13.12.2005)Google Scholar
  17. 17.
    Komiyama A, Umezawa I (1985) Acidic polysaccharide CH-1 isolated from Chlorella pyrenoidosa and the use thereof. Patent US No. 4533548 (06.08.1985)Google Scholar
  18. 18.
    Misra S, Toole BP, Ghatak S (2006) Hyaluronan constitutively regulates activation of multiple receptor tyrosine kinases in epithelial and carcinoma cells. J Bio Chem 281(46):34936CrossRefGoogle Scholar
  19. 19.
    Estrela-Llopis VR, Borodinova TI, Yurkova IN (2010) Extracellular biomineralization and synthesis of gold and platinum nano- and microcrystals in polysaccharide aqueous solutions, Chap. 12. In Starov V (ed) Nanoscience: colloidal and interfacial aspects. CRC Press, Boca Raton, pp 307–368CrossRefGoogle Scholar
  20. 20.
    Daba AS, Ezeronye OU (2003) Anti-cancer effect of polysaccharides isolated from higher basidiomycetes mushrooms. Afr J Biotechnol 2(12):672–678Google Scholar
  21. 21.
    Xu AH, Chen HS, Sun BC, Xiang XR, Chu YF, Zhai F, Jia LC (2003) Therapeutic mechanism of ginkgo biloba exocarp polysaccharides on gastric cancer. World J Gastroenterol 9(11):2424–2427Google Scholar
  22. 22.
  23. 23.
    Hodnett EM, Amirmoazzami J, Tai JTH (1978) Anionic polymers and biological activities: effects of some new polycarboxylic acids on the ascitic sarcoma 180 of mice. J Med Chem 21:7Google Scholar
  24. 24.
    Yessine MA, Leroux JC (2004) Membrane-destabilizing polyanions: interaction with lipid bilayers and endosomal escape of biomacromolecules. Adv Drug Deliv Rev 56:999–1021CrossRefGoogle Scholar
  25. 25.
    Yu W, Zhang N, Li C (2009) Saccharide modified pharmaceutical nanocarriers for targeted drug and gene delivery. Curr Pharm Des 15(32):3826–3836CrossRefGoogle Scholar
  26. 26.
    Shapot VS (1975) Biochemical aspects of tumor growth.Moscow: Medicine, 304 (rus)Google Scholar
  27. 27.
    Osinski S, Vaupel P (2009) Microphysiology of tumors. Kiev: Naukovadumka,108 (rus)Google Scholar
  28. 28.
    Hayat MA (2000) Principles and techniques of electron microscopy: biological applications. Cambridge University Press, Cambridge, p 543Google Scholar
  29. 29.
    Du Bois M., Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28(3):350CrossRefGoogle Scholar
  30. 30.
    Estrela-Llopis VR et al (2009) Investigation of coagulation gold and platinum nanoparticles and nanostructures in blood plasma and their interaction with transformed cells of certain tumorigenesis to be used in oncology therapy. Report on research work No. 0107U007693 (rus)Google Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • V. R. Estrela-Llopis
    • 1
    Email author
  • A. V. Chevichalova
    • 1
  • N. A. Tregubova
    • 2
  • E. D. Shishko
    • 2
  • P. M. Litvin
    • 3
  1. 1.F. D. Ovcharenko Institute of Biocolloid ChemistryUkrainian National Academy of SciencesKyivUkraine
  2. 2.R. E. Kavetsky Institute of Experimental Pathology, Oncology and RadiobiologyUkrainian National Academy of SciencesKyivUkraine
  3. 3.V. E. Lashkarev Institute of Semiconductor PhysicsUkrainian National Academy of SciencesKyivUkraine

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