Nanobiotechnologies in the System of Farm Animals’ Gene Pool Preservation

  • S. I. Kovtun
  • N. P. Galagan
  • O. V. ShcherbakEmail author
  • N. Y. Klymenko
  • O. S. Osypchuk
Conference paper
Part of the Springer Proceedings in Physics book series (SPPHY, volume 156)


It has been determined that sperm viability and mobility upon long storage in liquid nitrogen is being reduced. This issue is relevant for gene pool objects’ preservation and for reproductive medicine. The use of nanomaterials (NM) in reproductive biotechnology can expand to the methodology of the rational gene pool preservation technology, especially of valuable biomaterial. Use of nanoparticles, based on ultrafine silica (UFS) with different agents located on its surface gives a positive effect on the thawed sperm mobility, increasing its activity and survival rate and leading to increased fertilization ability. After thawing, the sperm of bulls had an average activity level of 47 %. This activity index of gametes in control was being maintained for 30 min. In the experimental groups after 30 min, the most active gametes were those with UFS + sucrose (52 %). A lower activity compared with the control and UFS + sucrose had gametes with UFS + D-galactosamine. Sperm activity with UFS + D-galactosamine decreased by 14 % compared with the control and by 19 % compared to the UFS + sucrose. So, the feasibility of adding NM with UFS + sucrose at a concentration of 0.001 % to thawed bull sperm that has been cryopreserved for a long time was proved.


Composite Surface Layer Sucrose Molecule Animal Genetic Resource High Temperature Hydrolysis Develop Surface Area 
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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • S. I. Kovtun
    • 1
  • N. P. Galagan
    • 2
  • O. V. Shcherbak
    • 1
    Email author
  • N. Y. Klymenko
    • 2
  • O. S. Osypchuk
    • 1
  1. 1.Biotechnology laboratory, Institute of Animal Breeding and GeneticsNational Academy of Agrarian Science of UkraineKievUkraine
  2. 2.O.O. Chuiko Institute of Surface ChemistryNational Academy of Science of UkraineKievUkraine

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