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Simplifying the Proteome: Analytical Strategies for Improving Peak Capacity

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 806))

Abstract

The diversity of biological samples and dynamic range of analytes being analyzed can prove to be an analytical challenge and is particularly prevalent to proteomic studies. Maximizing the peak capacity of the workflow employed can extend the dynamic range and increase identification rates. The focus of this chapter is to present means of achieving this for various analytical techniques such as liquid chromatography, mass spectrometry, and ion mobility. A combination of these methods can be used as part of a data-independent acquisition strategy, thereby limiting issues such as chimericy when analyzing regions of extreme analyte density.

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Acknowledgements

The authors would like to thank our collaborators who have provided figures and granted permission to use their collateral. In particular we would like to acknowledge Dr. Stefan Tenzer, Dr. Eva Rodriguez-Suárez, Dr. Sandra Kraljević Pavelić, Dr. Martin Gilar, Dr. John Shockcor, Kenneth Fountain, and Eric Grumbach. Finally Dr. Johannes P.C. Vissers is thanked for constructive comments during review.

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Authors and Affiliations

  1. Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, SK9 4AX, Manchester, UK

    Lee A. Gethings & Joanne B. Connolly

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  1. Lee A. Gethings
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  2. Joanne B. Connolly
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Correspondence to Lee A. Gethings .

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Editors and Affiliations

  1. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Alisa G. Woods

  2. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Costel C. Darie

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Gethings, L.A., Connolly, J.B. (2014). Simplifying the Proteome: Analytical Strategies for Improving Peak Capacity. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_3

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