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Using Proteomics to Unravel the Mysterious Steps of the HBV-Life-Cycle

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 806))

Abstract

Infection with Hepatitis B virus (HBV) is the most common cause of liver disease in the world. Infection becomes chronic in up to 10 % of adults, with severe consequences on liver function, including inflammation, fibrosis, cirrhosis, and eventually hepatocellular carcinoma (HCC). HCC is a fast progressing disease causing the death of approximately one million patients annually; current treatment has very limited success, mainly due to late-stage diagnosis and poor screening methodologies. Therefore, unraveling the complex HBV-host cell interactions during progression of the disease is of crucial importance, not only to understand the mechanisms underlying carcinogenesis, but importantly, for the development of new biomarkers for prognostic and early diagnosis. This is an area of research strongly influenced by proteomic studies, which have benefited in the last decade from major technical improvements in accuracy of quantification and sensitivity, large-scale analysis of low-abundant proteins, such as those from clinical samples being now possible and widely applied. This work is a critical review of the impact of the proteomic studies on our current understanding of HBV-associated pathogenesis, diagnostics, and treatment.

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Abbreviations

CID:

Collision-induced dissociation

HBV:

Hepatitis B virus

HCC:

Hepatocellular carcinoma

HCV:

Hepatitis C virus

m/z :

Mass/charge

MS:

Mass spectrometry

nanoLC-MS/MS:

Nanoliquid chromatography tandem mass spectrometry

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

TIC:

Total ion current

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Acknowledgements

The work was supported by the Romanian Academy project 3 of the Institute of Biochemistry and POSDRU/89/1.5/S/60746; Catalina Petrareanu was supported by the Sectoral Operational Programme Human Resources Development 2007–2013 of the Romanian Ministry of Labour, Family, and Social Protection through the Financial Agreement POSDRU/107/1.5/S/76903 grant.

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Authors and Affiliations

  1. Department of Viral Glycoproteins, Institute of Biochemistry of the Romanian Academy, Splaiul Independentei, 296, Sector 6, Bucharest, 060031, Romania

    Norica Branza-Nichita Ph.D., Catalina Petrareanu & Catalin Lazar

  2. Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Calea Grivitei, 132, Sector 1, Bucharest, 010707, Romania

    Norica Branza-Nichita Ph.D. & Catalina Petrareanu

  3. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699-5810, USA

    Izabela Sokolowska & Costel C. Darie

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  1. Norica Branza-Nichita Ph.D.
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  2. Catalina Petrareanu
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  3. Catalin Lazar
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  4. Izabela Sokolowska
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  5. Costel C. Darie
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Corresponding author

Correspondence to Norica Branza-Nichita Ph.D. .

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Editors and Affiliations

  1. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Alisa G. Woods

  2. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Costel C. Darie

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Branza-Nichita, N., Petrareanu, C., Lazar, C., Sokolowska, I., Darie, C.C. (2014). Using Proteomics to Unravel the Mysterious Steps of the HBV-Life-Cycle. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_22

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