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Mass Spectrometry-Based Tissue Imaging of Small Molecules

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 806))

Abstract

Mass spectrometry imaging (MSI) of tissue samples is a promising analytical tool that has quickly become associated with biomedical and pharmacokinetic studies. It eliminates several labor-intensive protocols associated with more classical imaging techniques and provides accurate histological data at a rapid pace. Because mass spectrometry is used as the readout, MSI can be applied to almost any molecule, especially those that are biologically relevant. Many examples of its utility in the study of peptides and proteins have been reported; here we discuss its value in the mass range of small molecules. We explore its success and potential in the analysis of lipids, medicinals, and metal-based compounds by featuring representative studies from MSI laboratories around the globe.

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Acknowledgments

This work was supported by the Ruth L. Kirschstein National Research Service Award (Grant No. GM007185, UCLA Cellular and Molecular Biology Training Grant, for C.N.F.) and the US National Institutes of Health Shared Instrumentation Program (Grant No. S10 RR025600 to J.A.L.).

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    Authors and Affiliations

    1. Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA

      Carly N. Ferguson, Joseph W. M. Fowler, Jonathan F. Waxer & Joseph A. Loo

    2. Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA

      Richard A. Gatti

    3. Department of Human Genetics, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA

      Richard A. Gatti

    4. Department of Biological Chemistry, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA

      Joseph A. Loo

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    1. Carly N. Ferguson
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    2. Joseph W. M. Fowler
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    3. Jonathan F. Waxer
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    4. Richard A. Gatti
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    Correspondence to Joseph A. Loo .

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    Editors and Affiliations

    1. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

      Alisa G. Woods

    2. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

      Costel C. Darie

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    Ferguson, C.N., Fowler, J.W.M., Waxer, J.F., Gatti, R.A., Loo, J.A. (2014). Mass Spectrometry-Based Tissue Imaging of Small Molecules. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_12

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