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Cryogels for Biotechnological Applications

  • Bo MattiassonEmail author
Chapter
Part of the Advances in Polymer Science book series (POLYMER, volume 263)

Abstract

Cryogels are formed in a semifrozen state when the solvent is frozen, but solutes are still soluble. The ice crystals are porogens and, upon thawing the system, pores appear where the frozen solvent was found earlier. Such gels have large pores, are elastic, and offer interesting opportunities in biotechnology. Cryogels with their large pores can meet demands that traditional chromatographic media cannot. This also opens up opportunities for the separation of cells because upon passage through the gel cells may interact with specific groups on the pore walls, thereby becoming retarded and/or captured. A range of applications have been studied: isolation of microbial cells, capturing of cancer cells, and use of cryogels as matrices for immobilized cell reactors. Furthermore, the robustness of the gels allows new applications, for example in environmental separation.

Keywords

Cell chromatography Immobilized cells Molecular imprinting Cell bioreactors Composite cryogels 

Abbreviations

AAm

Acrylamide

BSA

Bovine serum albumin

ECS

Extra capillary space

HFR

Hollow fiber reactor

HMs

Heavy metal ions

HSA

Human serum albumin

ICS

Intra capillary space

IDA

Immuno-diacetic-acid

MIP

Molecularly imprinted polymer

MPG

Macroporous gel particle

NIP

Non-imprinted polymer

NIPA

N-Isopropyl acrylamide

PAAm

Polyacrylamide

PEG

Polyethylene glycol

PHEMA

Poly(2-hydroxyethyl methacrylate)

PVA

Polyvinyl alcohol

SEM

Scanning electron microscope

Notes

Acknowledgements

This work was supported by The Swedish Research Council.

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  1. 1.Department of BiotechnologyLund UniversityLundSweden
  2. 2.Indienz AB, c/o Department of ChemistryLund UniversityLundSweden

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