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Solasodine Production in Solanum eleagnifolium in vitro Cultures

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Plant Biotechnology for Health

Abstract

Solanum eleagnifolium Cav. var leprosum (Ortega) Dunal is a species that grow in Argentina. It is characterized for accumulating, mainly in green fruits, the glycoalkaloid solasodine, the nitrogenated analogue of diosgenine, which can be used for the synthesis of steroidal compounds (e.g.: contraceptives and corticosteroids).

In vitro cultures of S. eleagnifolium Cav. were established, and the conditions for achieving high solasodine yields were studied. Plant growth regulators balance, the age and size of the inocula, the conduction of the productive process in undifferentiated cultures and in hairy roots and transformed stem cultures were studied. Also, an approach to large-scale culture was performed. Plant growth balance appeared as the most significant strategy for improving solasodine yields in undifferentiated in vitro cultures of S. eleagnifolium Cav.

On the other hand, the solasodine levels attained in hairy root cultures were the highest, demonstrating that differentiation is related to the amount of for solasodine produced.

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Authors and Affiliations

  1. School of Pharmacy and Biochemistry, CONICET/Universidad Maimónides, Buenos Aires, Argentina

    María Alejandra Alvarez

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  1. María Alejandra Alvarez
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Alvarez, M.A. (2014). Solasodine Production in Solanum eleagnifolium in vitro Cultures . In: Plant Biotechnology for Health. Springer, Cham. https://doi.org/10.1007/978-3-319-05771-2_5

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