Skip to main content

Dilute Acid Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse for Ethanol Production

  • Chapter
  • First Online:
Biofuels in Brazil

Abstract

Many efforts have been dedicated to understanding and refining different technologies to promote the conversion of the sugars contained in the sugarcane bagasse into ethanol. One of the promising strategies include the pretreatment of the bagasse with dilute sulfuric acid followed by the saccharification of the remaining polysaccharides with enzymes, and by the fermentation of the generated monosaccharides (both hexoses and pentoses) with yeasts. In the present chapter, data regarding the characterization and conditioning of the raw material as well as its pretreatment, saccharification, and fermentation are disclosed to illustrate that the sugarcane bagasse, like many other agroindustrial residues, consists of a heterogeneous material and that its different constituent sugars, not necessarily only plant cell wall polysaccharides, may need to be recovered under different experimental conditions if high conversion yields are to be achieved using the proposed technology.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Agbogbo FK, Coward-Kelly G (2008) Cellulosic ethanol production using the naturally occurring xylose-fermenting yeast Pichia stipitis. Biotechnol Lett 30:1515–1524

    Article  PubMed  CAS  Google Scholar 

  • Aguilar R, Ramírez JA, Garrote G, Vázquez M (2002) Kinetic study of the acid hydrolysis of sugar cane bagasse. J Food Eng 55:309–318

    Article  Google Scholar 

  • Akin DE (2007) Grass lignocellulose: strategies to overcome recalcitrance. Appl Biochem Biotechnol 136:3–15

    Article  Google Scholar 

  • Alves LA, Almeida e Silva JB, Giulietti M (2007) Solubility of d-glucose in water and ethanol/water mixtures. J Chem Eng Data 52:2166–2170

    Google Scholar 

  • Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101:4851–4861

    Article  PubMed  CAS  Google Scholar 

  • Andric P, Meyer AS, Jensen PA, Dam-Johansen K (2010) Effect and modeling of glucose inhibition and in situ glucose removal during enzymatic hydrolysis of pretreated wheat straw. Appl Biochem Biotechnol 160:280–297

    Article  PubMed  CAS  Google Scholar 

  • Bettiga M, Bengstsson O, Hahn-Hägerdal B, Gorwa-Grauslund MF (2009) Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal utilization pathway. Microb Cell Fact 8:1–12

    Article  CAS  Google Scholar 

  • Bower S, Wickramasinghe R, Nagle NJ, Schell DJ (2008) Modeling sucrose hydrolysis in dilute sulfuric acid solutions at pretreatment conditions for lignocellulosic biomass. Bioresour Technol 90:7354–7362

    Article  CAS  Google Scholar 

  • Bragatto J, Segato F, Cota J, Mello DB, Oliveira MM, Buckeridge MS, Squina FM, Driemeier C (2012) Insights on how the activity of an endoglucanase is affected by physical properties of insoluble celluloses. J Phys Chem B 116:6128–6136

    Article  PubMed  CAS  Google Scholar 

  • Browning B (1967) Methods of wood chemistry. Wiley, New York

    Google Scholar 

  • Cai BY, Ge JP, Ling HZ, Cheng KK, Ping WX (2012) Statistical optimization of dilute sulfuric acid pretreatment of corncob for xylose recovery and ethanol production. Biomass Bioenergy 36:250–257

    Article  CAS  Google Scholar 

  • Canilha L, Milagres AMF, Silva SS, Almeida e Silva JB, Felipe MGA, Rocha GJM, Ferraz A, Carvalho W (2009) Sacarificação da biomassa lignocelulósica através de pré-hidrólise ácida seguida por hidrólise enzimática: Uma estratégia de “desconstrução” da fibra vegetal. Rev Analytica 44:48–54

    Google Scholar 

  • Canilha L, Carvalho W, Felipe MGA, Almeida e Silva JB, Giulietti M (2010) Ethanol production from sugarcane bagasse hydrolysate using Pichia stipitis. Appl Biochem Biotechnol 161:84–92

    Google Scholar 

  • Canilha L, Santos VTO, Rocha GJM, Almeida e Silva JB, Giulietti M, Silva SS, Felipe MGA, Ferraz A, Milagres AMF, Carvalho W (2011) A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid. J Ind Microbiol Biotechnol 38:1467–1475

    Google Scholar 

  • Carvalho W, Batista MA, Canilha L, Santos JC, Converti A, Silva SS (2004a) Sugarcane bagasse hydrolysis with phosphoric and sulfuric acids and hydrolysate detoxification for xylitol production. J Chem Technol Biotechnol 79:1308–1312

    Article  CAS  Google Scholar 

  • Carvalho W, Santos JC, Canilha L, Almeida e Silva JB, Felipe MGA, Mancilha IM, Silva SS (2004b) A study on xylitol production from sugarcane bagasse hemicellulosic hydrolysate by Ca-alginate entrapped cells in a stirred tank reactor. Process Biochem 39:2135–2141

    Google Scholar 

  • Carvalho W, Canilha L, Silva SS (2007) Semi-continuous xylitol bioproduction in sugarcane bagasse hydrolysate: effect of nutritional supplementation. Braz J Pharm Sci 43:47–53

    CAS  Google Scholar 

  • Carvalho W, Ferraz A, Milagres AMF (2008) Clean-up and concentration of manganese peroxidases recovered during the biodegradation of Eucalyptus grandis by Ceriporiopsis subvermispora. Enzyme Microb Technol 43:193–198

    Article  CAS  Google Scholar 

  • Cenbio (2013) Centro Nacional de Referência em Biomassa. www.cenbio.iee.usp.br. Accessed 15 July 2013

  • Conab (2013) Companhia Nacional de Abastecimento. www.conab.gov.br. Accessed: 15 July2013

  • Driemeier C, Oliveira MM, Mendes FM, Gómez EO (2011) Characterization of sugarcane bagasse powders. Powder Technol 214:111–116

    Article  CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Ebringerová A (2006) Structural diversity and application potential of hemicelluloses. Macromol Symp 232:1–12

    Article  CAS  Google Scholar 

  • Ek M, Gellerstedt G, Henriksson G (2009) Pulp and paper chemistry and technology. Walter de Gruyter, Berlin

    Book  Google Scholar 

  • Fengel D, Wegener G (1989) Wood: chemistry, ultrastructure, reactions. Walter de Gruyter, Berlin

    Google Scholar 

  • Gámez S, González-Cabriales JJ, Ramírez JA, Garrote G, Vázquez M (2006) Study of the hydrolysis of sugar cane bagasse using phosphoric acid. J Food Eng 74:78–88

    Article  CAS  Google Scholar 

  • Gouveia ES, Nascimento RT, Souto-Maior AM, Rocha GJM (2009) Validação de metodologia para a caracterização química de bagaço de cana-de-açúcar. Quím Nova 32:1500–1503

    Article  CAS  Google Scholar 

  • Grohmann K, Torget R, Himmel M (1986) Optimization of dilute acid pretreatment of biomass. Biotechnol Bioeng Symp 15:59–80

    CAS  Google Scholar 

  • Han B, Carvalho W, Canilha L, Silva SS, Almeida e Silva JB, McMillan JD, Wickramasinghe R (2006) Adsorptive membranes vs. resins for acetic acid removal from biomass hydrolysates. Desalination 193:361–366

    Google Scholar 

  • Hatfield R, Fukushima RS (2005) Can Lignin be accurately measured? Crop Sci 45:832–839

    Article  CAS  Google Scholar 

  • Hernández-Salas JM, Villa-Ramírez MS, Veloz-Rendón JS, Rivera-Hernández RA, Plascencia-Espinosa MA, Tejo-Estrada SR (2009) Comparative hydrolysis and fermentation of sugarcane and agave bagasse. Bioresour Technol 100:1238–1245

    Article  PubMed  CAS  Google Scholar 

  • Hsu TC, Guo GL, Chen WH, Wang WS (2010) Effect of dilute acid pretreatment of rice straw on structural properties and enzymatic hydrolysis. Bioresour Technol 101:4907–4913

    Article  PubMed  CAS  Google Scholar 

  • Jeffries TW, Kurtzman CP (1994) Strain selection, taxonomy and genetics of xylose-fermenting yeasts. Enzyme Microb Technol 16:922–932

    Article  CAS  Google Scholar 

  • Kai Y (1991). Chemistry of extractives. In: Hon DNS, Shiraishi N (eds) Wood and cellulosic chemistry. Marcel Dekker, New York

    Google Scholar 

  • Kim SB, Lee YY (2002) Diffusion of sulfuric acid within lignocellulosic biomass particles and its impact on dilute-acid pretreatment. Bioresour Technol 83:165–171

    Article  PubMed  CAS  Google Scholar 

  • Kuhad RC, Gupta R, Khasaa YP, Singh A, Zhang YHP (2011) Bioethanol production from pentose sugars: current status and future prospects. Renew Sust Energ Rev 15:4950–4962

    Article  CAS  Google Scholar 

  • Linde M, Jakobsson EV, Galbe M, Zacchi G (2006) Steam pretreatment of dilute H2SO4-impregnated wheat straw and SSF with low yeast and enzyme loadings for ethanol production. Biomass Bioenergy 32:326–332

    Article  CAS  Google Scholar 

  • Neureiter M, Danner H, Thomasser C, Saidi B, Braun R (2002) Dilute acid hydrolysis of sugar cane bagasse at varying conditions. Appl Biochem Biotechnol 98:49–58

    Article  PubMed  Google Scholar 

  • Nguyen QA, Tucker MP, Keller FA, Eddy FP (2000) Two-stage dilute-acid pretreatment of softwoods. Appl Biochem Biotechnol 84:561–576

    Article  PubMed  Google Scholar 

  • Öhgren K, Bura R, Saddler J, Zacchi G (2007a) Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. Bioresour Technol 28:2503–2510

    Article  CAS  Google Scholar 

  • Öhgren K, Bura R, Lesnicki G, Saddler J, Zacchi G (2007b) A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover. Process Biochem 42:834–839

    Article  CAS  Google Scholar 

  • Palmqvist E, Hahn-Hagerdal B (2000) Fermentation of lignocellulosic hydrolysates II: inhibitors and mechanisms of inhibition. Bioresour Technol 74:25–33

    Article  CAS  Google Scholar 

  • Pietrobon VC, Monteiro RTR, Pompeu GB, Borges EP, Lopes ML, Amorim HV, Cruz H, Viégas EKD (2011) Enzymatic hydrolysis of sugarcane bagasse pretreated with acid or alkali. Braz Arch Biol Technol 54:229–233

    Article  CAS  Google Scholar 

  • Sanjuán R, Anzaldo J, Vargas J, Turrado J, Patt R (2001) Morphological and chemical composition of pith and fibers from mexican sugarcane bagasse. Holz Roh Werkst 59:447–450

    Article  Google Scholar 

  • Santos VTO, Esteves PJ, Milagres AMF, Carvalho W (2011) Characterization of commercial cellulases and their use in the saccharification of a sugarcane bagasse sample pretreated with dilute sulfuric acid. J Ind Microbiol Biotechnol 38:1089–1098

    Article  PubMed  CAS  Google Scholar 

  • Scordia D, Cosentino SL, Jeffries TW (2010) Second generation bioethanol production from Saccharum spontaneum L. ssp aegyptiacum (Willd.) Hack. Bioresour Technol 101:5358–5365

    Article  PubMed  CAS  Google Scholar 

  • Shi J, Ebrik MA, Wyman CE (2011) Sugar yields from dilute sulfuric acid and sulfur dioxide pretreatments and subsequent enzymatic hydrolysis of switchgrass. Bioresour Technol 102:8930–8938

    Article  PubMed  CAS  Google Scholar 

  • Siqueira G, Milagres AMF, Carvalho W, Koch G, Ferraz A (2011) Topochemical distribution of lignin and hydroxycinnamic acids in sugar-cane cell walls and its correlation with the enzymatic hydrolysis of polysaccharides. Biotechnol Biofuels 4:1–9

    Article  CAS  Google Scholar 

  • Sluiter A, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Determination of extractives in biomass. National Renewable Energy Laboratory. http://www.nrel.gov/docs/gen/fy08/42619.pdf. Accessed 13 Sept 2010

  • Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2010) Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory. http://www.nrel.gov/biomass/pdfs/42618.pdf. Accessed 13 Sept 2010

  • Söderstrom J, Plicher L, Galbe M, Zacchi G (2003) Two-step pretreatment of softwood by dilute H2SO4 impregnation for ethanol production. Biomass Bioenergy 24:457–486

    Article  CAS  Google Scholar 

  • Taherzadeh MJ, Karimi K (2007) Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials: a review. Bioresources 4:707–738

    Google Scholar 

  • Tewari JC, Malik K (2007) In situ laboratory analysis of sucrose in sugarcane bagasse using attenuated total reflectance spectroscopy and chemometrics. Int J Food Sci Tech 42:200–207

    Article  CAS  Google Scholar 

  • Venalainen M, Harju AM, Saranpaa P, Kainulainen P, Tiitta M, Velling P (2004) The concentration of phenolics in brown-rot decay resistant and susceptible Scots pine heartwood. Wood Sci Technol 38:109–118

    Article  CAS  Google Scholar 

  • Ximenes E, Kim Y, Mosier N, Dien B, Ladisch M (2010) Inhibition of cellulases by phenols. Enzyme Microb Technol 46:170–176

    Article  CAS  Google Scholar 

  • Yang SJ, Kataeva I, Hamilton-Brehm SD, Engle NL, Tschaplinski TJ, Doepkke C, Davis M, Westpheling J, Adams MWW (2009) Efficient degradation of lignocellulosic plant biomass, without pretreatment, by the thermophilic anaerobe Anaerocellum thermophilum DSM 6725. Appl Environ Microbiol 75:4762–4769

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Wyman CE (1994) Ethanol from lignocellulosic biomass: technology, economics, and opportunities. Bioresour Technol 50:3–16

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial support from Fapesp, CNPq, CAPES and USP is acknowledged. W. Carvalho is thankful to the many who have contributed to the evolution of the study.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Walter Carvalho .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Esteves, P.J., Santi, C., Carvalho, W. (2014). Dilute Acid Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse for Ethanol Production. In: da Silva, S., Chandel, A. (eds) Biofuels in Brazil. Springer, Cham. https://doi.org/10.1007/978-3-319-05020-1_10

Download citation

Publish with us

Policies and ethics