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Quantitative MALDI MS Using Ionic Liquid Matrices

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Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry

Abstract

Since the introduction of ionic liquid matrices (ILM) for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, manifold applications for a range of different substance classes (e.g., amino acids, peptides or proteins) were described. ILM are composed of an equimolar mixture of classically used acidic MALDI matrices and organic bases, which allows for an almost infinite number of combinations with different properties. A major advantage offered by many ILM compared to classical crystalline matrices is a highly homogeneous sample distribution, which is especially important in regard to quantification.

In general the concentration of an analyte can be determined based on the intensity of its corresponding ion in the mass spectrum (MS1 quantification). For this purpose the use of an internal standard with a high similarity to the analyte of interest is usually recommended. However, in certain cases it is possible to quantify the analyte without the use of an internal standard. Here we describe two exemplary applications of MALDI MS using ILM for the determination of enzyme activities.

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References

  • Armstrong DW, Zhang LK, He LF, Gross ML (2001) Ionic liquids as matrixes for matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem 73(15):3679–3686

    Article  CAS  PubMed  Google Scholar 

  • Berthod A, Crank JA, Rundlett KL, Armstrong DW (2009) A second-generation ionic liquid matrix-assisted laser desorption/ionization matrix for effective mass spectrometric analysis of biodegradable polymers. Rapid Commun Mass Spectrom 23(21):3409–3422

    Article  CAS  PubMed  Google Scholar 

  • Bungert D, Bastian S, Heckmann-Pohl DM, Giffhorn F, Heinzle E, Tholey A (2004) Screening of sugar converting enzymes using quantitative MALDI-ToF mass spectrometry. Biotechnol Lett 26(13):1025–1030

    Article  CAS  PubMed  Google Scholar 

  • Cohen LH, Gusev AI (2002) Small molecule analysis by MALDI mass spectrometry. Anal Bioanal Chem 373(7):571–586

    Article  CAS  PubMed  Google Scholar 

  • Cramer R, Corless S (2005) Liquid ultraviolet matrix-assisted laser desorption/ionization—mass spectrometry for automated proteomic analysis. Proteomics 5(2):360–370

    Article  CAS  PubMed  Google Scholar 

  • Crank JA, Armstrong DW (2009) Towards a second generation of ionic liquid matrices (ILMs) for MALDI-MS of peptides, proteins, and carbohydrates. J Am Soc Mass Spectrom 20(10):1790–1800

    Article  CAS  PubMed  Google Scholar 

  • de Rond T, Danielewicz M, Northen T (2015) High throughput screening of enzyme activity with mass spectrometry imaging. Curr Opin Biotechnol 31:1–9

    Article  PubMed  Google Scholar 

  • Distler AM, Allison J (2001) Improved MALDI-MS analysis of oligonucleotides through the use of fucose as a matrix additive. Anal Chem 73(20):5000–5003

    Article  CAS  PubMed  Google Scholar 

  • Dreisewerd K (2003) The desorption process in MALDI. Chem Rev 103(2):395–426

    Article  CAS  PubMed  Google Scholar 

  • Dreisewerd K (2014) Recent methodological advances in MALDI mass spectrometry. Anal Bioanal Chem 406(9–10):2261–2278

    Article  CAS  PubMed  Google Scholar 

  • Gabriel SJ, Pfeifer D, Schwarzinger C, Panne U, Weidner SM (2014) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric imaging of synthetic polymer sample spots prepared using ionic liquid matrices. Rapid Commun Mass Spectrom 28(5):489–498

    Article  CAS  PubMed  Google Scholar 

  • Giffhorn F (2000) Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl Microbiol Biotechnol 54(6):727–740

    Article  CAS  PubMed  Google Scholar 

  • Greis KD (2007) Mass spectrometry for enzyme assays and inhibitor screening: an emerging application in pharmaceutical research. Mass Spectrom Rev 26(3):324–339

    Article  CAS  PubMed  Google Scholar 

  • Gusev AI, Wilkinson WR, Proctor A, Hercules DM (1996) Direct quantitative analysis of peptides using matrix assisted laser desorption ionization. Anal Bioanal Chem 354(4):455–463

    Article  CAS  PubMed  Google Scholar 

  • Harvey DJ (1999) Matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates. Mass Spectrom Rev 18(6):349–450

    Article  CAS  PubMed  Google Scholar 

  • Hillenkamp F, Karas M, Beavis RC, Chait BT (1991) Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. Anal Chem 63(24):1193A–1203A

    Article  CAS  PubMed  Google Scholar 

  • Jaskolla TW, Lehmann WD, Karas M (2008) 4-Chloro-alpha-cyanocinnamic acid is an advanced, rationally designed MALDI matrix. Proc Natl Acad Sci U S A 105(34):12200–12205

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kang MJ, Tholey A, Heinzle E (2000) Quantitation of low molecular mass substrates and products of enzyme catalyzed reactions using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 14(21):1972–1978

    Article  CAS  PubMed  Google Scholar 

  • Karas M, Hillenkamp F (1988) Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 60(20):2299–2301

    Article  CAS  PubMed  Google Scholar 

  • Knochenmuss R, Zenobi R (2003) MALDI ionization: the role of in-plume processes. Chem Rev 103(2):441–452

    Article  CAS  PubMed  Google Scholar 

  • Lemaire R, Tabet JC, Ducoroy P, Hendra JB, Salzet M, Fournier I (2006) Solid ionic matrixes for direct tissue analysis and MALDI imaging. Anal Chem 78(3):809–819

    Article  CAS  PubMed  Google Scholar 

  • Li YL, Gross ML (2004) Ionic-liquid matrices for quantitative analysis by MALDI-TOF mass spectrometry. J Am Soc Mass Spectrom 15(12):1833–1837

    Article  CAS  PubMed  Google Scholar 

  • Mank M, Stahl B, Boehm G (2004) 2,5-Dihydroxybenzoic acid butylamine and other ionic liquid matrixes for enhanced MALDI-MS analysis of biomolecules. Anal Chem 76(10):2938–2950

    Article  CAS  PubMed  Google Scholar 

  • Nicola AJ, Gusev AI, Proctor A, Jackson EK, Hercules DM (1995) Application of the fast-evaporation sample preparation method for improving quantification of angiotensin II by matrix-assisted laser desorption/ionization. Rapid Commun Mass Spectrom 9(12):1164–1171

    Article  CAS  PubMed  Google Scholar 

  • Palmblad M, Cramer R (2007) Liquid matrix deposition on conductive hydrophobic surfaces for tuning and quantitation in UV-MALDI mass spectrometry. J Am Soc Mass Spectrom 18(4):693–697

    Article  CAS  PubMed  Google Scholar 

  • Reetz MT (2003) An overview of high-throughput screening systems for enantioselective enzymatic transformations. Methods Mol Biol 230:259–282

    CAS  PubMed  Google Scholar 

  • Sun P, Armstrong DW (2010) Ionic liquids in analytical chemistry. Anal Chim Acta 661(1):1–16

    Article  CAS  PubMed  Google Scholar 

  • Sze ET, Chan TW, Wang G (1998) Formulation of matrix solutions for use in matrix-assisted laser desorption/ionization of biomolecules. J Am Soc Mass Spectrom 9(2):166–174

    Article  CAS  PubMed  Google Scholar 

  • Tholey A (2006) Ionic liquid matrices with phosphoric acid as matrix additive for the facilitated analysis of phosphopeptides by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom 20(11):1761–1768

    Article  CAS  PubMed  Google Scholar 

  • Tholey A, Heinzle E (2002) Methods for biocatalyst screening. Adv Biochem Eng Biotechnol 74:1–19

    CAS  PubMed  Google Scholar 

  • Tholey A, Heinzle E (2006) Ionic (liquid) matrices for matrix-assisted laser desorption/ionization mass spectrometry-applications and perspectives. Anal Bioanal Chem 386(1):24–37

    Article  CAS  PubMed  Google Scholar 

  • Tholey A, Zabet-Moghaddam M, Heinzle E (2006) Quantification of peptides for the monitoring of protease-catalyzed reactions by matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrixes. Anal Chem 78(1):291–297

    Article  CAS  PubMed  Google Scholar 

  • Towers MW, McKendrick JE, Cramer R (2010) Introduction of 4-chloro-alpha-cyanocinnamic acid liquid matrices for high sensitivity UV-MALDI MS. J Proteome Res 9(4):1931–1940

    Article  CAS  PubMed  Google Scholar 

  • Vaidyanathan S, Gaskell S, Goodacre R (2006) Matrix-suppressed laser desorption/ionisation mass spectrometry and its suitability for metabolome analyses. Rapid Commun Mass Spectrom 20(8):1192–1198

    Article  CAS  PubMed  Google Scholar 

  • Wittmann C, Heinzle E (2001) MALDI-TOF MS for quantification of substrates and products in cultivations of Corynebacterium glutamicum. Biotechnol Bioeng 72(6):642–647

    Article  CAS  PubMed  Google Scholar 

  • Zabet-Moghaddam M, Heinzle E, Tholey A (2004) Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrices. Rapid Commun Mass Spectrom 18(2):141–148

    Article  CAS  PubMed  Google Scholar 

  • Zabet-Moghaddam M, Heinzle E, Lasaosa M, Tholey A (2006) Pyridinium-based ionic liquid matrices can improve the identification of proteins by peptide mass-fingerprint analysis with matrix-assisted laser desorption/ionization mass spectrometry. Anal Bioanal Chem 384(1):215–224

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. AG Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany

    Joanna Tucher, Prasath Somasundaram & Andreas Tholey

Authors
  1. Joanna Tucher
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  2. Prasath Somasundaram
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  3. Andreas Tholey
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Corresponding author

Correspondence to Andreas Tholey .

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Editors and Affiliations

  1. Department of Chemistry, University of Reading, Reading, Berkshire, United Kingdom

    Rainer Cramer

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Tucher, J., Somasundaram, P., Tholey, A. (2016). Quantitative MALDI MS Using Ionic Liquid Matrices. In: Cramer, R. (eds) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. Springer, Cham. https://doi.org/10.1007/978-3-319-04819-2_5

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