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Large Animal Induced Pluripotent Stem Cells as Models of Human Diseases

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Stem Cells in Animal Species: From Pre-clinic to Biodiversity

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

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Abstract

Pluripotent stem cells from the early embryos, the embryonic stem cells (ESC), have been the subject of intensive investigation for the past two decades. Much of the excitement surrounding ESC research has been centered on the potential of ESC in differentiating into target cell types, disease modeling, and in offering cures for intractable diseases. So far, rodents and primates are the only species from which “bonafide” ESC have been readily established and have met relatively stringent criteria of pluripotency. However, attempts to generate analogous pluripotent stem cells from other relevant domestic ungulates have been unsuccessful. While the reasons for poor outcome still remain a focus of active investigation, the establishment of induced pluripotent stem cells (iPSC) from somatic cells by upregulation of select few reprogramming factors has emerged as an attractive option. In the last few years since the inception, the iPSC field has witnessed substantial progress, with iPSC established from every major large animal species. The iPSC are playing an important role in understanding species-specific differences in pluripotency, unique requirements for their in vitro culture, and in achieving scientific and technical progress towards the establishment of authentic stem cell lines. These topics will be illustrated and discussed in detail in this chapter.

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Abbreviations

EpiSC:

Epiblast stem cells

ESC:

Embryonic stem cells

FGF:

Fibroblast growth factor

iPSC:

Induced pluripotent stem cells

LIF:

Leukemia inhibiting factor

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Authors and Affiliations

  1. Department of Animal and Avian Sciences, University of Maryland, 2121 ANSC Building, College Park, MD, 20742, USA

    Anjali Nandal & Bhanu Prakash V. L. Telugu

  2. Animal Bioscience and Biotechnology Laboratory, USDA Agricultural Research Service (USDA-ARS), Beltsville, MD, 20705, USA

    Anjali Nandal & Bhanu Prakash V. L. Telugu

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  1. Anjali Nandal
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  2. Bhanu Prakash V. L. Telugu
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Correspondence to Bhanu Prakash V. L. Telugu .

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Editors and Affiliations

  1. Università degli Studi di Milano, Laboratory of Biomedical Embriology; Unistem; Centre for Stem Cell Research, Milan, Italy

    Tiziana A.L. Brevini

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Nandal, A., Telugu, B.P.V.L. (2014). Large Animal Induced Pluripotent Stem Cells as Models of Human Diseases. In: Brevini, T. (eds) Stem Cells in Animal Species: From Pre-clinic to Biodiversity. Stem Cell Biology and Regenerative Medicine. Humana Press, Cham. https://doi.org/10.1007/978-3-319-03572-7_3

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