Efficient and Selective Alkane Hydroxylation Reactions Catalyzed by the Fungal Peroxygenase AaeAPOOpen image in new window
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In this chapter, we report AaeAPO catalyzed alkane hydroxylations with H2O2 as the sole oxidant. High selectivity for alcohols, high efficiency of H2O2 utilization, high regioselectivity and stereospecificity were observed. The scope of the alkane substrates includes linear, branched and cyclic hydrocarbons, further expanding to gaseous ethane, propane and neopentane. Metabolites of several drug molecules were also analyzed and compared with P450s metabolites. Combining with coenzymes or cofactors, AaeAPO can also utilize the environmentally desirable oxidant O2 to perform C–H oxidation. All these various reactions suggest that AaeAPO has potential practical application as an industrial biocatalyst.
KeywordsGluconic Acid Veratryl Alcohol Linear Alkane Potential Industrial Application Methylococcus Capsulatus
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