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Imaging Methods

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Part of the book series: Springer Theses ((Springer Theses))

Abstract

For widefield microscopy, a Leica DMIRB inverted microscope was used for fluorescence and phase contrast imaging of live E. coli cells.

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References

  1. M. Morphew, W. He, P.J. Bjorkman, J.R. McIntosh, Silver enhancement of nanogold particles during freeze substitution for electron microscopy. J. Microsc. 230(Pt 2), 263–267 (2008)

    Article  CAS  Google Scholar 

  2. W. He, C. Kivork, S. MacHinani, M. Morphew, A. Gail, D. Tesar, N. Tiangco, J. McIntosh, P. Bjorkman, A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed nanogold-labeled molecules. J. Struct. Biol. 160(1), 103–113 (2007)

    Article  CAS  Google Scholar 

  3. J.R. van Weering, E. Brown, T.H. Sharp, J. Mantell, P.J. Cullen, P. Verkade, Intracellular membrane traffic at high resolution. Methods Cell Biol. 96, 619–648 (2010)

    Article  Google Scholar 

  4. J.R. Kremer, D.N. Mastronarde, J.R. McIntosh, Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116(1), 71–76 (1996)

    Article  CAS  Google Scholar 

  5. B. Gipson, X. Zeng, Z. Zhang, H. Stahlberg, 2dx-User-friendly image processing for 2D crystals. J. Struct. Biol. 157(1), 64–72 (2007)

    Article  CAS  Google Scholar 

  6. M.D. Winn, C.C. Ballard, K.D. Cowtan, E.J. Dodson, P. Emsley, P.R. Evans, R.M. Keegan, E.B. Krissinel, A.G.W. Leslie, A. McCoy, S.J. McNicholas, G.N. Murshudov, N.S. Pannu, E.A. Potterton, H.R. Powell, R.J. Read, A. Vagin, K.S. Wilson, Overview of the CCP4 suite and current developments. Acta Crystallogr. Sect. D 67(4), 235–242 (2011)

    Article  CAS  Google Scholar 

  7. C.H. Owen, D.G. Morgan, D.J. DeRosier, Image analysis of helical objects: the Brandeis helical package. J. Struct. Biol. 116(1), 167–175 (1996)

    Article  CAS  Google Scholar 

  8. M. Stewart, Computer image processing of electron micrographs of biological structures with helical symmetry. J. Electron Microsc. Tech. 9(4), 325–358 (1988)

    Article  CAS  Google Scholar 

  9. G. Tang, L. Peng, P. Baldwin, D. Mann, W. Jiang, I. Rees, S. Ludtke, EMAN2: an extensible image processing suite for electron microscopy. J. Struct. Biol. 157(1), 38–46 (2007)

    Article  CAS  Google Scholar 

  10. G. Offer, R. Sessions, Computer modelling of the alpha-helical coiled coil: packing of side-chains in the inner core. J. Mol. Biol. 249(5), 967–987 (1995)

    Article  CAS  Google Scholar 

  11. R.D.B. Fraser, T.P. MacRae, G.E. Rogers, in Keratins: Their Composition, Structure, and Biosynthesis, ed. by C.C. Thomas (Springfield, Illinois, 1972)

    Google Scholar 

  12. Y. Harpaz, M. Gerstein, C. Chothia, Volume changes on protein folding. Structure 2(7), 641–649 (1994)

    Article  CAS  Google Scholar 

  13. T. Goddard, C. Huang, T. Ferrin, Visualizing density maps with UCSF chimera. J. Struct. Biol. 157(1), 281–287 (2007)

    Article  CAS  Google Scholar 

  14. E.F. Pettersen, T.D. Goddard, C.C. Huang, G.S. Couch, D.M. Greenblatt, E.C. Meng, T.E. Ferrin, UCSF chimera-a visualization system for exploratory research and analysis. J. Comput. Chem. 25(13), 1605–1612 (2004)

    Article  CAS  Google Scholar 

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Correspondence to Thomas Harry Sharp .

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Sharp, T.H. (2014). Imaging Methods. In: Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-02159-1_3

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