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Complexes Formation Between Proteins and Polyelectrolytes and Their Application in the Downstream Processes of Enzyme Purification

  • Guillermo Alfredo PicóEmail author
  • Nadia Woitovich Valetti
Chapter
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Part of the Engineering Materials book series (ENG.MAT.)

Abstract

Natural and synthetic polyelectrolytes have acquired notable importance in recent years due to their increasing application in different areas. One of these is downstream process methods which include the recovery, separation, concentration and purification of target enzymes from their natural sources. Polyelectrolytes interact with proteins to form soluble or non-soluble complexes. The interaction is driven by experimental variables of media such as pH, protein isoelectrical value, polyelectrolyte pKa, ionic strength and the presence of salts. The concentration of polyelectrolytes necessary to precipitate a protein completely is of the order of 10-4 – 10-2 % p/v. Precipitation of protein by PE is a novel technique integrating clarification, concentration and initial purification in a single step. This chapter presents some properties of aqueous solutions of natural and synthetic PE as a tool to use them in the protein downstream process.

Keywords

Isothermal Titration Calorimetry Target Enzyme Negative Electrical Charge Turbidimetric Titration Opposite Electrical Charge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

PE

Polyelectrolytes

PEC

Polyelectrolytes complex

PE-P

Polyelectrolytes-protein complexes

CAT

Catalase

Eu

Eudragit

PAA

Poly (acrylic acid)

PVS

Poly (vinylsulfonic acid)

Chy

Chymotrypsin

PEI

Polyethylenimine

Notes

Acknowledgments

This work was supported by a grant from CONICET PIP00196. We thank María Robson, Geraldine Raimundo, Mariana De Sanctis and Marcela Culasso for the language correction of the manuscript.

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Guillermo Alfredo Picó
    • 1
    • 2
    Email author
  • Nadia Woitovich Valetti
    • 2
  1. 1.National Scientific and Technical Research CouncilBuenos AiresArgentina
  2. 2.Biotechnological Process Laboratory, Faculty of Biochemical and Pharmaceutical SciencesNational University of RosarioRosarioArgentina

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