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Polymer Microflow Cytofluorometer

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Sensors and Microsystems

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 268))

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Abstract

This paper reports a microflow cytometer fabricated in polymethylmethacrylate (PMMA) in which a 3D hydrodynamic flow focusing is employed in order to align the particles in a single line along the focused stream. By exploiting an innovative and simple technique to realize a 3D hydrodynamic focusing effect, a tunable and circular-shaped focused stream has been obtained. The device has been fabricated by direct micromilling of two parts of PMMA that were finally bonded together. Flow cytometry measurements have been performed by using 10 μm-sized fluorescent particles. From the analysis of the fluorescence signals collected at each transit event, we can confirm that the device is capable of creating a single-file particle stream.

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References

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Author information

Authors and Affiliations

  1. Institute for Electromagnetic Monitoring of the Environment (IREA), National Research Council (CNR), Naples, Italy

    G. Testa, G. Persichetti & R. Bernini

Authors
  1. G. Testa
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  2. G. Persichetti
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  3. R. Bernini
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Corresponding author

Correspondence to G. Testa .

Editor information

Editors and Affiliations

  1. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  2. Department of Information Engineering, University of Brescia, Brescia, Italy

    Vittorio Ferrari

  3. SENSOR Laboratory, IDASC, National Research Council (CNR), Brescia, Italy

    Andrea Ponzoni

  4. Department of Information Engineering, University of Brescia, Brescia, Italy

    Giorgio Sberveglieri

  5. Department of Information Engineering, University of Brescia, Brescia, Italy

    Marco Ferrari

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© 2014 Springer International Publishing Switzerland

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Testa, G., Persichetti, G., Bernini, R. (2014). Polymer Microflow Cytofluorometer. In: Di Natale, C., Ferrari, V., Ponzoni, A., Sberveglieri, G., Ferrari, M. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 268. Springer, Cham. https://doi.org/10.1007/978-3-319-00684-0_42

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  • DOI: https://doi.org/10.1007/978-3-319-00684-0_42

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-00683-3

  • Online ISBN: 978-3-319-00684-0

  • eBook Packages: EngineeringEngineering (R0)

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