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Development and Characterization of a Novel Antibacterial Material Based on GOx Immobilized in a PVA Film

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Sensors and Microsystems

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 268))

Abstract

The development and characterization of a novel antibacterial material based on glucose oxidase (GOx) immobilized in a polyvinyl alcohol (PVA) film was proposed. This system acts in the presence of glucose to generate by-products as oxygen species (H2O2, ·O2 −, OH) that are well-known endogenous and exogenous toxic products for microbes in vivo (Miller and Britigan, Clin Microb R10: 1–18, 1997). The PVA/GOx composite material has been extensively characterized by X-ray photoelectron (XPS) spectroscopy, Fourier transform infrared (FTIR), and UV-visible spectroscopy (UV–vis) to verify the preservation of the enzyme structural integrity and of the enzymatic activity in PVA membrane. The antibacterial lysozyme-like activity of PVA/GOx was evaluated by a standard assay on Petri dishes employing Micrococcus lysodeikticus dried cell walls.

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Authors and Affiliations

  1. Università del Salento, via Monteroni, 73100, Lecce, Italy

    M. R. Guascito, D. Chirizzi & L. Giotta

  2. Istituto per l’Ambiente Marino Costiero CNR, via Roma 3, 74100, Taranto, Italy

    L. Stabili

Authors
  1. M. R. Guascito
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  2. D. Chirizzi
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  3. L. Giotta
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  4. L. Stabili
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Corresponding author

Correspondence to M. R. Guascito .

Editor information

Editors and Affiliations

  1. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  2. Department of Information Engineering, University of Brescia, Brescia, Italy

    Vittorio Ferrari

  3. SENSOR Laboratory, IDASC, National Research Council (CNR), Brescia, Italy

    Andrea Ponzoni

  4. Department of Information Engineering, University of Brescia, Brescia, Italy

    Giorgio Sberveglieri

  5. Department of Information Engineering, University of Brescia, Brescia, Italy

    Marco Ferrari

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© 2014 Springer International Publishing Switzerland

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Guascito, M.R., Chirizzi, D., Giotta, L., Stabili, L. (2014). Development and Characterization of a Novel Antibacterial Material Based on GOx Immobilized in a PVA Film. In: Di Natale, C., Ferrari, V., Ponzoni, A., Sberveglieri, G., Ferrari, M. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 268. Springer, Cham. https://doi.org/10.1007/978-3-319-00684-0_36

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  • DOI: https://doi.org/10.1007/978-3-319-00684-0_36

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-00683-3

  • Online ISBN: 978-3-319-00684-0

  • eBook Packages: EngineeringEngineering (R0)

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