Abstract
The use of ion-mobility spectrometry (IMS) coupled to mass spectrometry (IMS–MS) for biomolecule analyses has steadily increased over the past two decades, and is now applied to both proteomic and metabolomic investigations. This chapter describes the application of traveling-wave ion-mobility spectrometry–mass spectrometry (TWIMS–MS) to the analysis of a selection of bioactive phytochemicals used in dietary supplements. Applications include the analysis of grape seed proanthocyanidins and the structural characterization of bioactive constituents of dietary supplements using TWIMS-MS in conjunction with tandem mass spectrometry. We also discussed is the application of TWIMS-MS for the gas-phase mobility separation of structural isomers and the estimation of collision cross sections for a small selection of phenolic compounds from hop Recent applications of IMS–MS to a broad range of biomolecule measurements have demonstrated that IMS–MS has emerged as a powerful analytical technique capable of providing the separation space necessary to analyze highly complex samples. We give a perspective on emerging applications of IMS–MS for small molecule and biopolymer applications.The combination of devices that allow real-time monitoring of living systems using IMS–MS is an exciting avenue of facilitating system-biology experiments. The future of IMS–MS is bright and full of opportunities.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tsao R (2010) Chemistry and biochemistry of dietary polyphenols. Nutrients 2:1231–1246
Stevens JF, Page JE (2004) Xanthohumol and related prenylflavonoids from hops and beer: to your good health! Phytochemistry 65:1317–1330
Miranda CL et al (2012) Flavonoids. eLS, Wiley Online Library
Counterman AE, Clemmer DE (2002) Cis-trans signatures of proline-containing tryptic peptides in the gas phase. Anal Chem 74:1946–1951
Kaleta DT, Jarrold MF (2003) Helix-turn-helix motifs in unsolvated peptides. J Am Chem Soc 125:7186–7187
Ruotolo BT et al (2004) Ion mobility-mass spectrometry applied to cyclic peptide analysis: conformational preferences of gramicidin S and linear analogs in the gas phase. J Am Soc Mass Spectrom 15:870–878
Ruotolo BT et al (2002) Observation of conserved solution-phase secondary structure in gas-phase tryptic peptides. J Am Chem Soc 124:4214–4215
Ruotolo BT et al (2005) Evidence for macromolecular protein rings in the absence of bulk water. Science 310:1658–1661
Uetrecht C et al (2010) Ion mobility mass spectrometry of proteins and protein assemblies. Chem Soc Rev 39:1633–1655
Zilch LW et al (2007) Folding and unfolding of helix-turn-helix motifs in the gas phase. J Am Soc Mass Spectrom 18:1239–1248
Trimpin S, Clemmer DE (2008) Ion mobility spectrometry/mass spectrometry snapshots for assessing the molecular compositions of complex polymeric systems. Anal Chem 80:9073–9083
Trimpin S et al (2007) Resolving oligomers from fully grown polymers with IMS-MS. Anal Chem 79:7965–7974
Dear GJ et al (2010) Sites of metabolic substitution: investigating metabolite structures utilising ion mobility and molecular modelling. Rapid Commun Mass Spectrom 24:3157–3162
Cuyckens F et al (2011) Product ion mobility as a promising tool for assignment of positional isomers of drug metabolites. Rapid Commun Mass Spectrom 25:3497–3503
Dong L et al (2010) Collision cross-section determination and tandem mass spectrometric analysis of isomeric carotenoids using electrospray ion mobility time-of-flight mass spectrometry. Anal Chem [Epub ahead of print]
Bohrer BC, Clemmer DE (2011) Biologically-inspired peptide reagents for enhancing IMS-MS analysis of carbohydrates. J Am Soc Mass Spectrom 22:1602–1609
Dwivedi P et al (2010) Metabolic profiling of human blood by high resolution ion mobility mass spectrometry (IM-MS). Int J Mass Spectrom 298:78–90
Castro-Perez J et al (2011) Localization of fatty acyl and double bond positions in phosphatidylcholines using a dual stage CID fragmentation coupled with ion mobility mass spectrometry. J Am Soc Mass Spectrom 22:1552–1567
Kliman M et al (2011) Lipid analysis and lipidomics by structurally selective ion mobility-mass spectrometry. Biochim Biophys Acta 1811:935–945
Kanu AB et al (2008) Ion mobility-mass spectrometry. J Mass Spectrom 43:1–22
Verbeck GF et al (2002) A fundamental introduction to ion mobility mass spectrometry applied to the analysis of biomolecules. J Biomol Tech 13:56–61
Bohrer BC et al (2008) Biomolecule analysis by ion mobility spectrometry. Annu Rev Anal Chem (Palo Alto Calif) 1:293–327
Liu X et al (2007) Mapping the human plasma proteome by SCX-LC-IMS-MS. J Am Soc Mass Spectrom 18:1249–1264
Giles K et al (2004) Applications of a travelling wave-based radio-frequency-only stacked ring ion guide. Rapid Commun Mass Spectrom 18:2401–2414
Giles K et al (2011) Enhancements in travelling wave ion mobility resolution. Rapid Commun Mass Spectrom 25:1559–1566
Michaelevski I et al (2010) T-wave ion mobility-mass spectrometry: basic experimental procedures for protein complex analysis. J Vis Exp 41: e1985
Smith DP KT, Campuzano I, Malham RW, Berryman JT, Radford SE, Ashcroft AE (2009) Deciphering drift time measurements from travelling wave ion mobility spectrometry-mass spectrometry studies. Eur J Mass Spectrom (Chichester Eng) 15:113–130
Monagas M et al (2010) MALDI-TOF MS analysis of plant proanthocyanidins. J Pharm Biomed Anal 51:358–372
Mouls L et al (2011) Comprehensive study of condensed tannins by ESI mass spectrometry: average degree of polymerisation and polymer distribution determination from mass spectra. Anal Bioanal Chem 400:613–623
Taylor AW et al (2003) Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography. J Agric Food Chem 51:4101–4110
Porter PJ (1988) Flavans and proanthocyanidins. The Flavonoids 21–62
Aron PM, Kennedy JA (2008) Flavan-3-ols: nature, occurrence and biological activity. Mol Nutr Food Res 52:79–104
Hayasaka Y et al (2003) Characterization of proanthocyanidins in grape seeds using electrospray mass spectrometry. Rapid Commun Mass Spectrom 17:9–16
Harry EL et al (2009) Direct analysis of pharmaceutical formulations from non-bonded reversed-phase thin-layer chromatography plates by desorption electrospray ionisation ion mobility mass spectrometry. Rapid Commun Mass Spectrom 23:2597–2604
Williams JP et al (2009) Isomer separation and gas-phase configurations of organoruthenium anticancer complexes: ion mobility mass spectrometry and modeling. J Am Soc Mass Spectrom 20:1119–1122
http://www.indiana.edu/~clemmer/Research/Cross%20Section%20Database/Peptides/polyaminoacid_cs.htm.Accessed 14 Oct 2013
http://www.indiana.edu/~nano/index.html.Accessed 14 Oct 2013
Wyttenbach T et al (1997) Effect of the long-range potential on ion mobility measurements. J Am Soc Mass Spectrom 8:275–282
Ruotolo BT et al (2008) Ion mobility-mass spectrometry analysis of large protein complexes. Nat Protoc 3:1139–1152
Knapman TW et al. (2010) Determining the topology of virus assembly intermediates using ion mobility spectrometry-mass spectrometry. Rapid Commun Mass Spectrom 24:3033–3042
Kennedy JA, Jones GP (2001) Analysis of proanthocyanidin cleavage products following acid-catalysis in the presence of excess phloroglucinol. J Agric Food Chem 49:1740–1746
Salbo R et al (2012) Traveling-wave ion mobility mass spectrometry of protein complexes: accurate calibrated collision cross-sections of human insulin oligomers. Rapid Commun Mass Spectrom 26:1181–1193
Li H et al (2012) Resolving structural isomers of monosaccharide methyl glycosides using drift tube and traveling wave ion mobility mass spectrometry. Anal Chem 84:3231–3239
Goodwin CR et al (2012) Structural mass spectrometry: rapid methods for separation and analysis of peptide natural products. J Nat Prod 75:48–53
Rand K et al (2011) ETD in a traveling wave ion guide at tuned Z-spray ion source conditions allows for site-specific hydrogen/deuterium exchange measurements. J Am Soc Mass Spectrom 22:1784–1793
Halgand F et al (2011) Dividing to unveil protein microheterogeneities: traveling wave ion mobility study. Anal Chem 83:7306–7315
Ridenour WB et al (2010) Structural characterization of phospholipids and peptides directly from tissue sections by MALDI traveling-wave ion mobility-mass spectrometry. Anal Chem 82:1881–1889
Dwivedi P et al (2010) Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source. J Mass Spectrom 45:1383–1393
Kaplan K et al (2009) Monitoring dynamic changes in lymph metabolome of fasting and fed rats by electrospray ionization-ion mobility mass spectrometry (ESI-IMMS). Anal Chem 81:7944–7953
Enders JR et al (2010) Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform. IET Systems Biology 4:416–427
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Maier, C., Zandkarimi, F., Wickramasekara, S., Morre, J., Stevens, J. (2013). Electrospray Ionization Traveling Wave Ion Mobility Spectrometry Mass Spectrometry for the Analysis of Plant Phenolics: An Approach for Separation of Regioisomers. In: Gang, D. (eds) 50 Years of Phytochemistry Research. Recent Advances in Phytochemistry, vol 43. Springer, Cham. https://doi.org/10.1007/978-3-319-00581-2_2
Download citation
DOI: https://doi.org/10.1007/978-3-319-00581-2_2
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-00580-5
Online ISBN: 978-3-319-00581-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)