Abstract
The membranes of the endoplasmic reticulum (ER) represent the site of co-translational vectorial transfer of de novo synthesized proteins from the cytosol to the ER and of lipid biosynthesis. After translocation, post-translational protein modifications occur in the cisternal lumen.
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References
Alder NN, Shen Y, Brodsky JL, Hendershot LM, and Johnson AE (2005) The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum. J Cell BioI 168: 389
Burda P and Aebi M (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426: 239
Chavan M and Lennarz W (2006) The molecular basis of coupling of translocation and N-glycosylation. Trends Biochem Sci 31: 17
Johnson AE (2009) The structural and functional coupling of two molecular machines, the ribosome and the translocon. J Cell Biol 185:765
Knauer R and Lehle L (1999) The oligosaccharyltransferase complex from yeast. Biochim Biophys Acta 1426: 259
Menetret JF, Hegde RS, Heinrich SU, Chandramouli P, Ludtke SJ, Rapoport TA, and Akey CW (2005) Architecture of the ribosomechannel complex derived from native membranes. J Mol Biol 348: 445
Osborne AR, Rapoport TA, and van den Berg B (2005) Protein translocation by the Sec61/SecYchannel. Annu Rev Cell Dev Biol 21:529
Silberstein S and Gilmore R (1996) Biochemistry, molecular biology, and genetics of the oligosaccharyltransferase. FASEB J 10: 849
van den Berg B, Clemons WM, Collinson I, Modis Y, Hartmann E, Harrison SC, and Rapoport TA (2004) X-ray structure of a protein-conducting channel. Nature 427: 36
Zuber Z and Roth J (2009) N-glycosylation. In: The Sugar Code. Fundamentals of Glycosciences (Gabius H-J, ed). Weinheim: Wiley/VCH, pp 87
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Pavelka, M., Roth, J. (2010). Rough Endoplasmic Reticulum: Site of Protein Translocation and Initiation of Protein N-Glycosylation. In: Functional Ultrastructure. Springer, Vienna. https://doi.org/10.1007/978-3-211-99390-3_19
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DOI: https://doi.org/10.1007/978-3-211-99390-3_19
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-99389-7
Online ISBN: 978-3-211-99390-3
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