Abstract
Screening for new anticancer agents has traditionally been done with in vitro cell culture methods. Even in the genomic era of target-driven drug design, screening for cytotoxic activity is still a standard tool in the search for new anticancer agents, especially if the mode of action of a substance is not yet known. A wide variety of cell culture methods with unique end-points are available for testing the anticancer potential of a substance. Each has its advantages and disadvantages, which must be weighed in the decision to use a particular method. Often several complementary methods are used to gain information on the mode of action of a substance.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Freshney RI. Culture of animal cells. Hoboken, NJ: Wiley-Liss; 2005.
Pagé M. In: Cancer Therapeutics: Experimental and Clinical Agents, Ed. Teicher BA. Totowa, NJ: Humana. 1997.
Wilson AP. In: Animal Cell Culture: A Practical Approach, Ed. Freshney RI. Oxford: IRL. 1986; p. 183.
Boyd MR. In: Cancer Therapeutics: Experimental and Clinical Agents, Ed. Teicher BA. Totowa, NJ: Humana. 1997; p. 23.
Saotome K, Morita H, Umeda M. Toxic In Vitro. 1989;3:317.
Bracht K, Boubakari R, Grünert P, Bednarski J. Anti-Cancer Drugs. 2006;17:41.
Reile H, Birnböck H, Bernhardt G, Spruß T, Schönenberger H. Anal Biochem. 1990;187:262.
Blank M, Shiloh Y. Cell Cycle. 2007;6:686.
Fisher DM, Bednarski PJ, Grünert R, Turner P, Fenton RR, Aldrich-Wright JR. ChemMedChem. 2007;2:488.
Krause-Heuer AM, Grünert R, Kuehne S, Buczkowska M, Wheate NJ, Le Pevelen DD, et al. J Med Chem. 2009;52:5474.
Murray A, Hunt T. The cell cycle. Oxford: Oxford University Press; 1993.
Morasca L, Erba E. In: Animal Cell Culture: A Practical Approach, Ed. Freshney RI. Oxford: IRL. 1986; p. 125.
Facompré M, Wattez N, Kluza J, Lansiaux A, Bailly C. Mol Cell Biol Commun. 2000;4:37.
Eastman A. In: Cisplatin: Chemistry and Biochemistry of a Leading Anticancer Drug, Ed. Lippert B. Weinheim: Wiley-VCH. 1999, p. 111.
Acknowledgements
We wish to thank Erika Böttcher for technical assistance with the cell culture work and Kerstin Gumm for assisting in the flow cytometry measurements.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer-Verlag/Wien
About this chapter
Cite this chapter
Grünert, R., Westendorf, A., Buczkowska, M., Hänsch, M., Grüunert, S., Bednarski, P.J. (2011). Cytotoxicity Testing: Cell Experiments. In: Aldrich-Wright, J. (eds) Metallointercalators. Springer, Vienna. https://doi.org/10.1007/978-3-211-99079-7_14
Download citation
DOI: https://doi.org/10.1007/978-3-211-99079-7_14
Published:
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-09427-3
Online ISBN: 978-3-211-99079-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)