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The Use of Calorimetry to Study Ligand–DNA Interactions

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Abstract

Calorimetry monitors heat changes accompanying biological or chemical processes. As applied to the study of ligand–DNA interactions, calorimetry can be used to monitor processes including the isothermal titration of DNA targets with putative DNA binding ligands; as well as, the thermal denaturation of DNA in the absence and presence of binding ligands. By monitoring the small heat changes accompanying these events calorimetry can directly provide thermodynamic information which is invaluable for understanding the basis of how molecules interact with each other. In this chapter, the use of calorimetric approaches to the study of ligand–DNA interactions will be discussed.

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Authors and Affiliations

  1. Biophysical Core Facility, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA

    Nichola C. Garbett

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  1. Nichola C. Garbett
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Correspondence to Nichola C. Garbett .

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Editors and Affiliations

  1. School of Biomedical & Health Sciences, University of Western Sydney, Penrith South DC, New South Wales, Australia

    Janice Aldrich-Wright

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Garbett, N.C. (2011). The Use of Calorimetry to Study Ligand–DNA Interactions. In: Aldrich-Wright, J. (eds) Metallointercalators. Springer, Vienna. https://doi.org/10.1007/978-3-211-99079-7_12

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