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Effects of VEGF Administration or Neutralization on the BBB of Developing Rat Brain

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Brain Edema XIV

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 106))

Abstract

We investigated the effects of exogenous Vascular Endothelial Growth Factor VEGF combined with an enriched environment on BBB integrity after a minimal trauma induced during the first days of the critical visual period in rats, when peak levels of endogenous VEGF secretion are reached. VEGF was administered using osmotic mini-pumps placed in middle cortical layers of P18 Long-Evansrats. Tissue changes were evaluated using conventional histology. BBB integrity was shown by immunohistochemistry techniques for EBA and GluT-1. Mini-pump implantation produced a wider cavity in anti-VEGF infused rats. In VEGF-infused rats there was a damaged region around the cannula that was smaller in rats raised in an enriched environment (EE). The administration of VEGF induced a high concentration of plasma proteins in the neuropil around the point of cannula placement and a high inflammatory reaction. VEGF-infused rats raised in an EE showed a lower degree of extravasation and better tissue preservation. Anti-VEGF administration produced a lower protein expression profile and more widespread deterioration of tissue. Double immunofluorescence for EBA and GluT-1 showed that the administration of VEGF preserves the tissue, which remains present but not fully functional. In contrast, a combination of VEGF administration and an EE partially protects the functionally damaged tissue with a higher preservation of BBB integrity.

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Acknowledgements

This work was financially supported by 9/212.327-15887 (University of the Basque Country), IT/460/07 and SAIOTEK (Basque Government).

N. Ortuzar is supported by a scholarship from the University of the Basque Country.

Confocal microscopy analysis was performed at the “Servicio de Microscopía Analítica y de Alta Resolución en Biomedicina” (High Resolution Analytical Microscopy Service for Biomedicine) at the University of the Basque Country.

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Authors and Affiliations

  1. Clinical and Experimental Neuroscience Laboratory, Department of Neuroscience, University of Basque Country, Leioa, Spain

    J. V. Lafuente

  2. Department of Neuroscience, LaNCE; Clinical and Experimental Neuroscience Laboratory, University of Basque Country, Leioa, Spain

    N. Ortuzar, E. G. Argandoña, H. Bengoetxea, O. Leis & S. Bulnes

Authors
  1. N. Ortuzar
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  2. E. G. Argandoña
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  3. H. Bengoetxea
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  4. O. Leis
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  5. S. Bulnes
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  6. J. V. Lafuente
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Corresponding author

Correspondence to J. V. Lafuente .

Editor information

Editors and Affiliations

  1. Medical Research Center, PAN Warszawa, Ceglowska St. 80, Warszawa, 01-809, Poland

    Zbigniew Czernicki

  2. Klinikum Großhadern, Universitätsklinikum München, Marchioninistr. 15, München, 81377, Germany

    Alexander Baethmann

  3. Dept. Neurosurgery, Musashino Red Cross Hospital, Kyonan-cho 1-26-1, Musashino City, Tokyo, 180, Japan

    Umeo Ito

  4. School of Medicine, Nihon University, Oyaguchikami-machi 30-1 , Tokyo, 173-8610, Japan

    Yoichi Katayama

  5. Dept. Neuropathology, Medical Research Institute, Yushima 1-5-45, Tokyo, 113-8510, Japan

    Toshihiko Kuroiwa

  6. Dept. Neurosurgery, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne, NE46BE, United Kingdom

    David Mendelow

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Ortuzar, N., Argandoña, E.G., Bengoetxea, H., Leis, O., Bulnes, S., Lafuente, J.V. (2010). Effects of VEGF Administration or Neutralization on the BBB of Developing Rat Brain. In: Czernicki, Z., Baethmann, A., Ito, U., Katayama, Y., Kuroiwa, T., Mendelow, D. (eds) Brain Edema XIV. Acta Neurochirurgica Supplementum, vol 106. Springer, Vienna. https://doi.org/10.1007/978-3-211-98811-4_9

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  • DOI: https://doi.org/10.1007/978-3-211-98811-4_9

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-98758-2

  • Online ISBN: 978-3-211-98811-4

  • eBook Packages: MedicineMedicine (R0)

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